Plastidic RNA polymerase σ factors in Arabidopsis

被引:62
作者
Kanamaru, K [1 ]
Fujiwara, M
Seki, M
Katagiri, T
Nakamura, M
Mochizuki, N
Nagatani, A
Shinozaki, K
Tanaka, K
Takahashi, H
机构
[1] Univ Tokyo, Inst Mol & Cellular Biosci, Mol Genet Lab, Bunkyo Ku, Tokyo 1130032, Japan
[2] RIKEN, Inst Phys & Chem Res, Plant Mol Biol Lab, Tsukuba, Ibaraki 3050074, Japan
[3] Kyoto Univ, Dept Bot, Lab Plant Physiol, Sakyo Ku, Kyoto 6068502, Japan
基金
日本科学技术振兴机构;
关键词
Arabidopsis thaliana; chloroplast; plastid-encoded RNA polymerase (PEP); sigma factor;
D O I
10.1093/oxfordjournals.pcp.a029612
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In plant cells, plastid DNA is transcribed by at least two types of RNA polymerase, plastid-encoded RNA polymerase (PEP) and nuclear-encoded RNA polymerase (NEP), PEP is homologous to eubacterial transcription machinery, but its regulatory subunit, sigma (sigma) factor, is not encoded on the plastid DNA, We previously cloned the three nuclear-encoded sigma factor genes from Arabidopsis thaliana and designated them as sigA, sigB, and sigC. By means of RFLP mapping, sigA and sigB,were mapped on chromosome I and sigC on the chromosome III. Based on comparison of the genomic structure of the three sig genes, intron sites in the 3' half of the genes were shown to be identical between sigB and sigC but divergent in sigA, consistent with the phylogenetic relevance of the three gene products. A transient expression assay of GFP fusions in Arabidopsis protoplasts showed that the N-termini of all three sig gene products functioned as chloroplast-targeting signals. We also constructed transgenic Arabidopsis lines harboring the sigA-promoter or the sigB-promoter uidA fusion. Both the sigA- and sigB-promoters were similarly activated at cotyledons, hypocotyls, rosette leaves, cauline leaves, sepals, and siliques but not at roots, seeds, or other Bower organs. In addition, the two promoters were repeatedly activated in young seedlings under continuous light, possibly in an oscillated fashion.
引用
收藏
页码:832 / 842
页数:11
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