Sequence-specific electrochemical detection of Alicyclobacillus acidoterrestris DNA using electroconductive polymer-modified fluorine tin oxide electrodes

被引:21
作者
Barrios Eguiluz, Katlin Ivon [1 ]
Salazar-Banda, Giancarlo Richard [1 ]
Funes-Huacca, Maribel Elizabeth [2 ]
Alberice, Juliana Vieira [2 ]
Carrilho, Emanuel [2 ]
Spinola Machado, Sergio Antonio [1 ]
Avaca, Luis Alberto [1 ]
机构
[1] Univ Sao Paulo, GMEME, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil
[2] Univ Sao Paulo, BioMicS Grp Bioanalit Microfabricacao & Separacoe, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil
关键词
AMPEROMETRIC DETECTION; ORANGE JUICE; SOLAR-CELLS; HYBRIDIZATION; SENSOR; FILMS; PCR; AMPLIFICATION; NANOPARTICLES; BIOSENSORS;
D O I
10.1039/b809080g
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This study outlines the quantification of low levels of Alicyclobacillus acidoterrestris in pure cultures, since this bacterium is not inactivated by pasteurization and may remain in industrialized foods and beverages. Electroconductive polymer-modified fluorine tin oxide (FTO) electrodes and multiple nanoparticle labels were used for biosensing. The detection of A. acidoterrestris in pure cultures was performed by reverse transcription polymerase chain reaction (RT-PCR) and the sensitivity was further increased by asymmetric nested RT-PCR using electrochemical detection for quantification of the amplicon. The quantification of nested RT-PCR products by Ag/Au-based electrochemical detection was able to detect 2 colony forming units per mL (CFU mL(-1)) of spores in pure culture and low detection and quantification limits (7.07 and 23.6 nM, respectively) were obtained for the target A. acidoterrestris on the electrochemical detection bioassay.
引用
收藏
页码:314 / 319
页数:6
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