A new self-assembled modified electrode for competitive immunoassay

被引：26

作者：

Danilowicz, C ^{[1
]}

Manrique, JM ^{[1
]}

机构：

[1] Fac Farm & Bioquim, Dept Quim Analit & Fisicoquim, AR-1113 Buenos Aires, DF, Argentina

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 1999年 / 1卷 / 01期

关键词：

immunoassays; bio-electrodes; multilayers; immunosensors; redox polymers;

D O I：

10.1016/S1388-2481(98)00003-4

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Alternating films constructed upon successive deposition of redox polymer Os(bpy)(2)ClPyCH(2)NHpoly (allylamine) and antibiotin IgG were developed for amperometric immunosensor design. Cyclic voltammetric measurements were used to verify charge transport between redox sites and the redox surface concentration was estimated upon voltammetric peak integration. Biotin-antibiotin complex formation was evaluated using horseradish peroxidase as an enzyme label. Redox mediation between the modified electrode surface and the redox site in the enzyme was verified after substrate addition. Multilayer modified gold electrodes with biologically active antibiotin IgG molecules were employed for the development of a competitive immunoassay with electrochemical label detection. (C) 1999 Elsevier Science S.A. All rights reserved.

引用

页码：22 / 25

页数：4

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