Induction of germline transcription in the TCRγ locus by Stat5:: Implications for accessibility control by the IL-7 receptor

被引:106
作者
Ye, SK
Maki, K
Kitamura, T
Sunaga, S
Akashi, K
Domen, J
Weissman, IL
Honjo, T
Ikuta, K [1 ]
机构
[1] Kyoto Univ, Fac Med, Dept Med Chem, Kyoto 6068501, Japan
[2] Univ Tokyo, Inst Med Sci, Dept Hematopoiet Factors, Tokyo 1080071, Japan
[3] Tokyo Univ Hosp, Dept Hematol & Oncol, Tokyo 1138655, Japan
[4] Stanford Univ, Sch Med, Dept Pathol, Stanford, CA 94305 USA
[5] Stanford Univ, Sch Med, Dept Dev Biol, Stanford, CA 94305 USA
关键词
D O I
10.1016/S1074-7613(00)80096-5
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
IL-7 receptor (IL-7R) plays critical roles in lymphocyte development by promoting survival and proliferation and by inducing V(D)J recombination in TCR and Ig loci. Here, we demonstrate that IL-7R-activated Stat5 binds to consensus motifs in the 5' regions of J gamma segments and induces germline transcripts. We also show that a constitutively active form of Stat5 restores V-J recombination of TCR gamma genes and partially rescues T cell development from IL-7R(-/-) T cell precursors, especially in favor of gamma delta T cells. Therefore, this study reveals a potential role of Stat5 in T cell development and also implies that IL-7R may control the accessibility of the TCR gamma locus through Stat5-induced germline transcription.
引用
收藏
页码:213 / 223
页数:11
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