The process of pharynx regeneration in planarians

被引:50
作者
Kobayashi, C [1 ]
Watanabe, K [1 ]
Agata, K [1 ]
机构
[1] Himeji Inst Technol, Fac Sci, Dept Life Sci, Lab Regenerat Biol, Himeji, Hyogo 6781297, Japan
关键词
planarian; pharynx regeneration; pattern formation; cell commitment; pharynx muscle; pharynx epithelia; blastema;
D O I
10.1006/dbio.1999.9291
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
To understand the cellular events during planarian regeneration, we analyzed the process of pharynx regeneration in both head and tail pieces using cell-type-specific markers. Interestingly, cells expressing the pharynx-muscle-specific myosin heavy chain gene (DjMHC-A) appeared within 24 h after amputation (prior to the formation of a pharynx rudiment) in the mesenchymal space of the stump, not in the blastema region. These DjMHC-A-positive cells migrated to the midline and formed the pharynx rudiment. Even after formation of the pharynx rudiment, DjMHC-A-positive cells constantly appeared in the mesenchymal space in the region surrounding the pharynx rudiment and participated in the growth of the pharynx rudiment. These observations clearly indicated that the cells involved in pharynx-muscle formation are committed in the mesenchymal space of the stump, rather than in the blastema region or the pharynx rudiment during planarian regeneration. We also analyzed the process of regeneration of the pharynx epithelia using a monoclonal antibody and investigated the origin of the pharynx epithelia. (C) 1999 Academic Press.
引用
收藏
页码:27 / 38
页数:12
相关论文
共 31 条
[1]   Structure of the planarian central nervous system (CNS) revealed by neuronal cell markers [J].
Agata, K ;
Soejima, Y ;
Kato, K ;
Kobayashi, C ;
Umesono, Y ;
Watanabe, K .
ZOOLOGICAL SCIENCE, 1998, 15 (03) :433-440
[2]  
ASAI E, 1991, ZOOL SCI, V8, P775
[3]   THE BEHAVIOR OF PHARYNGEAL OUTER EPITHELIAL-CELLS DURING REGENERATION OF THE PLANARIAN DUGESIA-JAPONICA-JAPONICA [J].
ASAI, E .
JOURNAL OF MORPHOLOGY, 1990, 206 (03) :313-325
[4]   PLANARIAN NEOBLASTS [J].
BAGUNA, J ;
SLACK, JMW .
NATURE, 1981, 290 (5801) :14-15
[5]   MITOSIS IN INTACT AND REGENERATING PLANARIAN DUGESIA-MEDITERRANEA N-SP .1. MITOTIC STUDIES DURING GROWTH, FEEDING AND STARVATION [J].
BAGUNA, J .
JOURNAL OF EXPERIMENTAL ZOOLOGY, 1976, 195 (01) :53-64
[6]  
BAGUNA J, 1989, DEVELOPMENT, V107, P77
[7]  
BAGUNA J, 1994, ZOOL SCI, V11, P781
[8]  
BRONSTED HV, 1969, PLANARIAN REGENERATI
[9]   Cell-, tissue-, and position-specific monoclonal antibodies against the planarian Dugesia (Girardia) tigrina [J].
Bueno, D ;
Baguna, J ;
Romero, R .
HISTOCHEMISTRY AND CELL BIOLOGY, 1997, 107 (02) :139-149
[10]   TCEN-49, A MONOCLONAL-ANTIBODY THAT IDENTIFIES A CENTRAL BODY ANTIGEN IN THE PLANARIAN DUGESIA (GIRARDIA) TIGRINA - IMPLICATIONS FOR PATTERN-FORMATION AND POSITIONAL SIGNALING MECHANISMS [J].
BUENO, D ;
ESPINOSA, L ;
SORIANO, MA ;
BATLLE, E ;
BAGUNA, J ;
ROMERO, R .
HYDROBIOLOGIA, 1995, 305 (1-3) :235-240