Parameters optimization of chitin hydrolysis by Trichoderma harzianum chitinase under assay conditions

A kinetic analysis and optimization of reaction conditions for the enzymatic hydrolysis of chitin using chitinase produced by Trichoderma harzianum NCIM 1185 was carried out. Swollen chitin was used as the substrate for chitinase. The central composite design was followed for this optimization. The required volume ratio of the major reactants for maximum hydrolysis was determined. The pH and temperature optima were found to be 4.75 and 47 degrees C respectively. K-m and V-max for this enzyme were 4.643 kg/m(3) and 0.1542 U respectively.