Cryo-electron microscopy structure of yeast Ty retrotransposon virus-like particles

被引：21

作者：

Palmer, KJ

Tichelaar, W

Myers, N

Burns, NR

Butcher, SJ

Kingsman, AJ

Fuller, SD

Saibil, HR

机构：

[1] UNIV LONDON BIRKBECK COLL, DEPT CRYSTALLOG, LONDON WC1E 7HX, ENGLAND

[2] BRITISH BIOTECH PHARMACEUT LTD, OXFORD, ENGLAND

[3] UNIV OXFORD, DEPT BIOCHEM, OXFORD OX1 3QU, ENGLAND

[4] EUROPEAN MOL BIOL LAB, D-69012 HEIDELBERG, GERMANY

来源：

JOURNAL OF VIROLOGY | 1997年 / 71卷 / 09期

关键词：

D O I：

10.1128/JVI.71.9.6863-6868.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The virus like particles (VLPs) produced by the yeast retrotransposon Ty1 are functionally related to retroviral cores. These particles are unusual in that they have variable radii. A paired mass-radius analysis of VLPs by scanning transmission electron microscopy showed that many of these particles farm an icosahedral T-number series. Three-dimensional reconstruction to 38-Angstrom resolution from cryo-electron micrographs of T=3 and T=4 shells revealed that the single structural protein encoded by the TYA gene assembles into spiky shells from trimeric units.

引用

收藏

页码：6863 / 6868

页数：6

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