Protective effect of bioaccessible fractions of citrus fruit pulps against H2O2-induced oxidative stress in Caco-2 cells

被引：46

作者：

Cilla, Antonio ^{[1
]}

Rodrigo, Maria J. ^{[2
]}

Zacarias, Lorenzo ^{[2
]}

De Ancos, Begona ^{[3
]}

Sanchez-Moreno, Concepcion ^{[3
]}

Barbera, Reyes ^{[1
]}

Alegria, Amparo ^{[1
]}

机构：

[1] Univ Valencia, Fac Pharm, Nutr & Food Sci Area, Valencia, Spain

[2] CSIC, IATA, Valencia, Spain

[3] CSIC, Inst Food Sci Technol & Nutr ICTAN, Madrid, Spain

来源：

FOOD RESEARCH INTERNATIONAL | 2018年 / 103卷

关键词：

Citrus fruit pulps; Bioactive compounds; Antioxidants; in vitro digestion; Caco-2; cells; Oxidative stress; CAROTENOID BIOSYNTHESIS; ANTIOXIDANT CAPACITY; INDUCED CYTOTOXICITY; POSTHARVEST STORAGE; BETA-CRYPTOXANTHIN; DNA-DAMAGE; ORANGE; JUICE; CARA; SUPPLEMENTATION;

D O I：

10.1016/j.foodres.2017.10.066

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Fruit pulps from Navel (N) and Cara Cara (CC) oranges, and Clementine mandarin freshly harvested (M) and refrigerated stored (M12) were used to evaluate the cytoprotective effect of their bioaccessible fractions (BF) against H2O2-induced oxidative stress in Caco-2 cells. BF of samples preserved viability vs. H2O2 treated cells, reaching values similar to controls. Lipid peroxidation was reduced to levels of control cells, but M did not reach control values. ROS and mitochondrial membrane potential changes (Delta psi(m)) values were reduced compared with H2O2 treated cells, but without achieving control levels. A significant reduction in cell proportions in G1 phase and a significant increase in sub-G1 phase (apoptosis) of cell cycle was shown in H2O2 treated cells, and BF allowed a recovery close to control levels. Thus, BF of samples protect the cells from oxidative stress by preserving cell viability, mitochondrial membrane potential and correct cell cycle progression, and diminishing lipid peroxidation and ROS.

引用

页码：335 / 344

页数：10

共 44 条

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