Chemotaxonomy of bacteria by comprehensive GC and GC-MS in electron impact and chemical ionisation mode

被引：22

作者：

David, Frank ^{[1
]}

Tienpont, Bart ^{[1
]}

Sandra, Pat ^{[1
]}

机构：

[1] Res Inst Chromatog, B-8500 Kortrijk, Belgium

来源：

JOURNAL OF SEPARATION SCIENCE | 2008年 / 31卷 / 19期

关键词：

Bacterial fatty acid methyl esters; Chemotaxonomy; GC x GC; GC-MS; One-dimensional GC;

D O I：

10.1002/jssc.200800215

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The analysis of the cellular lipidic fraction of bacteria is described. After hydrolysis and methylation, the fatty acid methyl esters (FAMEs) are determined by 1-D GC using the Sherlock MIDI bacteria identification system, by comprehensive GC (GC x GC) and by GC-MS in electron impact (EI) and positive chemical ionisation (PCI) mode. With GC x GC, the enhanced selectivity and group type separation provides a more complete elucidation of the fatty acids in microorganisms. GC-EI-MS and GC-PCI-MS were helpful for confirmation. The bacteria selected in this study are Brevundimonas diminuta, Chryseobacterium gleum and Stenotrophomonas maltophilia.

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收藏

页码：3395 / 3403

页数：9

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