Laccase electrode for direct electrocatalytic reduction of O2 to H2O with high-operational stability and resistance to chloride inhibition

被引：136

作者：

Vaz-Dominguez, Cristina ^{[1
]}

Campuzano, Susana ^{[1
]}

Rudiger, Olaf ^{[1
]}

Pita, Marcos ^{[1
]}

Gorbacheva, Marina ^{[2
]}

Shleev, Sergey ^{[2
,3
]}

Fernandez, Victor M. ^{[1
]}

De lacey, Antonio L. ^{[1
]}

机构：

[1] CSIC, Inst Catalisis, Madrid 28049, Spain

[2] INBI, AN Bakh Biochem Inst, Moscow 119071, Russia

[3] Malmo Univ, S-20506 Malmo, Sweden

来源：

BIOSENSORS & BIOELECTRONICS | 2008年 / 24卷 / 04期

基金：

瑞典研究理事会;

关键词：

Laccase; Cathode; Biofuel cells; Direct electron transfer;

D O I：

10.1016/j.bios.2008.05.002

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Laccase from Trametes hirsuta basidiomycete has been covalently bound to graphite electrodes electrochemically modified with phenyl derivatives as a way to attach the enzyme molecules with an adequate orientation for direct electron transfer(DET). Current densities up to 0.5 mA/cm(2) of electrocatalytic reduction of O-2 to H2O were obtained in absence of redox mediators, suggesting preferential orientation of the T1 Cu centre of the laccase towards the electrode. The covalent attachment of the laccase molecules to the functionalized electrodes permitted remarkable operational stability. Moreover, O-2 bioelectroreduction based on DET between the laccase and the electrode was not inhibited by chloride ions, whereas mediated bioelectrocatalysis was. In contrast, fluoride ions inhibited both direct and mediated electron transfers-based bioelectrocatalytic reduction of O-2. Thus, two different modes of laccase inhibition by halides are discussed. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：531 / 537

页数：7

共 48 条

[1] Covalent modification of carbon surfaces by aryl radicals generated from the electrochemical reduction of diazonium salts [J].