A novel PHB depolymerase from a thermophilic Streptomyces sp.

被引：59

作者：

Calabia, BP ^{[1
]}

Tokiwa, Y ^{[1
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Tsukuba, Ibaraki 3058566, Japan

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 06期

关键词：

bacterial polyesters; poly(3-hydroxybutyrate) degrading enzyme; Streptomyces sp; synthetic polyesters; thermophilic;

D O I：

10.1007/s10529-005-6063-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel PHB depolymerase from a thermophilic Streptomyces sp. MG was purified to homogeneity by hydrophobic interaction chromatography and gel filtration. The molecular mass of the purified enzyme was 43 kDa as determined by size exclusion chromatography and 41 kDa by SDS-PAGE. The optimum pH and temperature were 8.5 and 60 degrees C respectively. The enzyme was stable at 50 degrees C and from pH 6.5-8.5. The enzyme hydrolyzed not only bacterial polyesters, i.e. poly(3-hydroxybutyric acid and poly(3-hydroxybutyrate-co-3-hydroxyvalerate), but also synthetic, aliphatic polyesters such as polypropiolactone, poly(ethylene adipate) and poly(ethylene succinate).

引用

页码：383 / 388

页数：6

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