Determination of proteins at nanogram levels with their enhancement effects of resonance lightscattering on quercetin

被引：35

作者：

Feng, P ^{[1
]}

Hu, XL ^{[1
]}

Huang, CZ ^{[1
]}

机构：

[1] SW Normal Univ, Inst Environm Chem, Chongqing 400715, Peoples R China

来源：

ANALYTICAL LETTERS | 1999年 / 32卷 / 07期

基金：

中国国家自然科学基金;

关键词：

proteins; quercetin; resonance light-scattering;

D O I：

10.1080/00032719908542900

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A determination method of proteins with the limit of determination at nanogram levels is proposed by using a common spectrofluorometer to detect intensity of resonance light-scattering (RLS). Proteins, including bovine serum albumin (BSA), human serum albumin (HSA), pepsin (Pep), alpha-chymotrypsin (Chy), lysozyme (Lys) and cellulase (Cel), can combine with quercetin (QT) in the pH range 4.10-4.35, and produce an enhanced RLS signal at 400.0 and 470.0 nn. Optimization conditions for the protein-QT interaction were tested. Depending on the concentration of QT, different proteins in the range 0 similar to 4.5 mu g ml(-1) can be determined with the limit df determination at nanogram levels.

引用

页码：1323 / 1338

页数：16

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