Distribution of mefE and ermB genes in macrolide-resistant strains of Streptococcus pneumoniae and their variable susceptibility to various antibiotics

被引:58
作者
Nishijima, T
Saito, Y
Aoki, A
Toriya, M
Toyonaga, Y
Fujii, R
机构
[1] Asahi Chem Ind Co Ltd, Life Sci Res Labs, Shizuoka 4102321, Japan
[2] Yamanashi Red Cross Hosp, Dept Pediat, Yamanashi, Japan
[3] Chemotherapy Mothers & Children Res Inst, Toshima Ku, Tokyo, Japan
关键词
D O I
10.1093/jac/43.5.637
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
From February to October 1995, 62 erythromycin-resistant strains of Streptococcus pneumoniae isolated at Yamanashi Red Cross Hospital were tested to determine their susceptibility to various macrolides, subjected to resistance induction tests by the disc diffusion method and analysed for genes encoding resistance to macrolides (ermB and mefE). On the basis of resistance induction testing, the isolates were classified as having either inducible (59.7%) or non-inducible (40.3%) macrolide resistance. The ermB gene was always detected in resistance-inducible type isolates, either alone or in combination with mefE. The mefE gene alone was found only in non-inducible type isolates. Isolates with non-inducible resistance (those with only the mefE gene) had an intermediate level of resistance to 14-membered macrolides, and were susceptible to rokitamycin, a 16-membered macrolide. According to NCCLS guidelines, 9.6% of S. pneumoniae strains were judged to be susceptible to penicillin, 62.9% of reduced susceptibility and 27.4% penicillin resistant. No correlation was detected between the presence of particular macrolide-resistance genes (ermB, ermB + mefE, or mefE) and resistance to penicillin G.
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页码:637 / 643
页数:7
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