Spatio-temporal relationship between nuclear-envelope breakdown and preprophase band disappearance in cultured tobacco cells

被引：30

作者：

Dixit, R ^{[1
]}

Cyr, RJ ^{[1
]}

机构：

[1] Penn State Univ, Dept Biol, Mueller Lab 208, University Pk, PA 16802 USA

来源：

PROTOPLASMA | 2002年 / 219卷 / 1-2期

关键词：

preprophase bands; nuclear-envelope breakdown; prometaphase; green-fluorescent protein; red-fluorescent protein;

D O I：

10.1007/s007090200012

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cell division involves the coordinated progression of karyokinesis and cytokinesis. which is accomplished by communication between the nucleus and the cytoplasm. We hake utilized green-fluorescent-protein technology to generate a line (A tobacco Bright Yellow 2' (BY-2) cells labeled for both microtubules and the nuclear envelope. This cell line allowed us to use living cells to investigate the relationship between nuclear-envelope breakdown and preprophase hand disappearance with high spatial and temporal resolution. Our observations demonstrate that nuclear-en elope breakdown always precedes preprophase band disappearance in BY-2 cells. In addition, the rate of preprophase band disappearance. and the attenuation of perinuclear microtubule fluorescence, correlates with the proximity of the nucleus to the preprophase band These results indicate the presence of communication between the nucleus and the preprophase band and suggest a causal relationship between nuclear-em elope breakdown and preprophase hand disappearance.

引用

页码：116 / 121

页数：6

共 20 条

[1]

CLEARY AL, 1992, J CELL SCI, V103, P977

[2]

COLASANTI J, 1993, PLANT CELL, V5, P1101

[3]

DIXIT R, 2001, IN PRESS PLANT J

[4] MAF1, a novel plant protein interacting with matrix attachment region binding protein MFP1, is located at the nuclear envelope [J].

Gindullis, P ;

Peffer, NJ ;

Meier, I .

PLANT CELL, 1999, 11 (09) :1755-1767

[5] Microtubule reorganization in tobacco BY-2 cells stably expressing GFP-MBD [J].

Granger, CL ;

Cyr, RJ .

PLANTA, 2000, 210 (03) :502-509

[6]

Granger CL, 2001, J CELL SCI, V114, P599

[7] Plant mitosis promoting factor disassembles the microtubule preprophase band and accelerates prophase progression in Tradescantia [J].

Hush, J ;

Wu, LP ;

John, PCL ;

Hepler, LH ;

Hepler, PK .

CELL BIOLOGY INTERNATIONAL, 1996, 20 (04) :275-287

[8] The preprophase band of microtubules: Its function as a cytokinetic apparatus in higher plants [J].

Mineyuki, Y .

INTERNATIONAL REVIEW OF CYTOLOGY - A SURVEY OF CELL BIOLOGY, VOL 187, 1999, 187 :1-49

[9] RELATIONSHIP BETWEEN THE PREPROPHASE BAND, NUCLEUS AND SPINDLE IN DIVIDING ALLIUM COTYLEDON CELLS [J].

MINEYUKI, Y ;

MARC, J ;

PALEVITZ, BA .

JOURNAL OF PLANT PHYSIOLOGY, 1991, 138 (06) :640-649

[10] Cyclin-dependent kinases: Engines, clocks, and microprocessors [J].

Morgan, DO .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1997, 13 :261-291

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