Short Term Effects of Doxycycline on Matrix Metalloproteinases 2 and 9

被引：21

作者：

Fiotti, Nicola ^{[1
]}

Altamura, Nicola ^{[1
]}

Moretti, Michele ^{[1
]}

Wassermann, Stella ^{[1
]}

Zacchigna, Serena ^{[2
]}

Farra, Rossella ^{[1
]}

Dapas, Barbara ^{[1
]}

Consoloni, Lara ^{[1
]}

Giacca, Mauro ^{[2
]}

Grassi, Gabriele ^{[3
]}

Giansante, Carlo ^{[1
]}

机构：

[1] Univ Trieste, Dipartimento Sci Clin Morfol & Tecnol, SC Clin Med Gen Terapia Med, I-34149 Trieste, Italy

[2] ICGEB, I-34012 Trieste, Italy

[3] Univ Trieste, Dipartimento Sci Vita, I-34125 Trieste, Italy

来源：

CARDIOVASCULAR DRUGS AND THERAPY | 2009年 / 23卷 / 02期

关键词：

Doxycycline; Metalloproteinase-9; Neutrophils degranulation; Nitrates; ABDOMINAL AORTIC-ANEURYSMS; DOUBLE-BLIND; EXPRESSION; NEUTROPHIL; MATRIX-METALLOPROTEINASE-9; INHIBITION; ACTIVATION; SERUM; PROCOLLAGENASE; RUPTURE;

D O I：

10.1007/s10557-008-6150-7

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

To investigate the short term effects of Doxycycline on MMP-2 and MMP-9. Short term effects of Doxycycline (100 mg B.I.D.) on plasma levels of MMP-2 and MMP-9 were investigated in 20 healthy subjects; the effects of Doxy, Acetylsalicylic acid, Nitrates, and Enalapril on MMP-9 release from were assessed in isolated polymorphonuclear cells. In plasma, MMP-9 activity was reduced (-22%, 95% CI -32/-11; P = 0.002) starting at 12 h after doxy; in vitro, MMP-9 released from stimulated neutrophils was reduced by Doxy (-28%, 95% CI -43/-14; P = 0.001), inhibiting degranulation, and by nitrates (-52%, 95% CI -76/-28 P = 0.005), increasing three times both pro- and active-MMP-9 bound to neutrophils (P = 0.007 and 0.040, respectively). Doxy decreases MMP-9 plasma levels by around 20%, within the first 12 h. The mechanism leading to such reduction seems due to the inhibition of PMN degranulation.

引用

页码：153 / 159

页数：7

共 40 条

[1] Prospective, randomized, double-blind trial investigating the effect of doxycycline on matrix metalloproteinase expression within atherosclerotic carotid plaques [J].

Axisa, B ;

Loftus, IM ;

Naylor, AR ;

Goodall, S ;

Jones, L ;

Bell, PRF ;

Thompson, MM .

STROKE, 2002, 33 (12) :2858-2864

[2] Prolonged administration of doxycycline in patients with small asymptomatic abdominal aortic aneurysms: Report of a prospective (Phase II) multicenter study [J].

Baxter, BT ;

Pearce, WH ;

Waltke, EA ;

Littooy, FN ;

Hallett, JW ;

Kent, KC ;

Upchurch, GR ;

Chaikof, EL ;

Mills, JL ;

Fleckten, B ;

Longo, GM ;

Lee, JK ;

Thompson, RW .

JOURNAL OF VASCULAR SURGERY, 2002, 36 (01) :1-12

[3]

BELLAVITE P, 1983, EUR J CLIN INVEST, V13, P363

[4] PROTEOLYTIC REMODELING OF EXTRACELLULAR-MATRIX [J].

BIRKEDALHANSEN, H .

CURRENT OPINION IN CELL BIOLOGY, 1995, 7 (05) :728-735

[5] Clinical and biochemical results of the metalloproteinase inhibition with subantimicrobial doses of doxycycline to prevent acute coronary syndromes (MIDAS) pilot trial [J].

Brown, DL ;

Desai, KK ;

Vakili, BA ;

Nouneh, C ;

Lee, HM ;

Golub, LM .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2004, 24 (04) :733-738

[6]

BURNS FR, 1989, INVEST OPHTH VIS SCI, V30, P1569

[7] Nitric oxide donors selectively reduce the expression of matrix metalloproteinases-8 and -9 by human diabetic skin fibroblasts [J].

Burrow, Jamey W. ;

Koch, Joey A. ;

Chuang, Hui-Hsiu ;

Zhong, Weinan ;

Dean, David D. ;

Sylvia, Victor L. .

JOURNAL OF SURGICAL RESEARCH, 2007, 140 (01) :90-98

[8] Captopril, an angiotensin-converting enzyme inhibitor, attenuates the severity of acute pancreatitis in rats by reducing expression of matrix metalloproteinase 9 [J].

Chen, Ping ;

Yuan, Yaozong ;

Wang, Shiwen ;

Zhan, Leheng ;

Xu, Jiayu .

TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, 2006, 209 (02) :99-107

[9] Preoperative treatment with doxycycline reduces aortic wall expression and activation of matrix metalloproteinases in patients with abdominal aortic aneurysms [J].

Curci, JA ;

Mao, DL ;

Bohner, DG ;

Allen, BT ;

Rubin, BG ;

Reilly, JM ;

Sicard, GA ;

Thompson, RW .

JOURNAL OF VASCULAR SURGERY, 2000, 31 (02) :325-341

[10] The pro and the active form of matrix metalloproteinase-9 is increased in serum of patients with amyotrophic lateral sclerosis [J].

Demestre, M ;

Parkin-Smith, G ;

Petzold, A ;

Pullen, AH .

JOURNAL OF NEUROIMMUNOLOGY, 2005, 159 (1-2) :146-154

← 1 2 3 4 →