Characterization of unusual G8 rotavirus strains isolated from Egyptian children

被引:64
作者
Holmes, JL
Kirkwood, CD
Gerna, G
Clemens, JD
Rao, MR
Naficy, AB
Abu-Elyazeed, R
Savarino, SJ
Glass, RI
Gentsch, JR
机构
[1] Ctr Dis Control & Prevent, Viral Gastroenteritis Sect MS G04, Div Viral & Rickettsial Dis,Nat Ctr Infect Dis, Publ Hlth Serv,US Dept Hlth & Human Serv, Atlanta, GA 30333 USA
[2] Policlin San Matteo, IRCCS, Viral Diagnost Serv, I-27100 Pavia, Italy
[3] NICHHD, Epidemiol Branch, Bethesda, MD 20892 USA
[4] USN, Med Res Unit 3, Cairo, Egypt
关键词
D O I
10.1007/s007050050594
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We report the first detection of P[14], G8 rotaviruses isolated in Egypt from the stool of children participating in a 3 year study of rotavirus epidemiology. Two strains, EGY1850 and EGY2295, were characterized by a serotyping enzyme immunoassay (EIA), virus neutralization, and sequence analysis of the genes encoding VP7 and the VP8* portion of the VP4 gene. These two strains shared a high level of homology of their VP7s (87.8% nucleotide [nt], 97.2% amino acid [aa]) and VP4s (89.6% nt, 97.1% aa) and had the highest VP7 identity to serotype G8 (>82% nt, >92% aa) and VP4 identity to genotype P[14] (greater than or equal to 81% nt, >91% aa) strains. Serological results with a VP7 G8-specific and VP4 P[14]-specific neutralizing monoclonal antibodies supported the genetic classification of EGY1850 and EGY2295 as P[14], G8. Genogroup analysis supports earlier findings that human G8 rotaviruses may be genetically related to bovine rotaviruses. These findings demonstrate that our understanding of the geographic distribution of rotavirus strains is incomplete, emphasize the need to monitor rotavirus serotypes, and extend the known distribution of serotype G8 and genotype P[14] strains in Africa.
引用
收藏
页码:1381 / 1396
页数:16
相关论文
共 59 条
  • [1] Nigerian rotavirus serotype GS could not be typed by PCR due to nucleotide mutation at the 3' end of the primer binding site
    Adah, MI
    Rohwedder, A
    Olaleyle, OD
    Werchau, H
    [J]. ARCHIVES OF VIROLOGY, 1997, 142 (09) : 1881 - 1887
  • [2] CULTIVATION AND CHARACTERIZATION OF HUMAN ROTAVIRUSES WITH SUPER SHORT RNA PATTERNS
    ALBERT, MJ
    UNICOMB, LE
    BISHOP, RF
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (01) : 183 - 185
  • [3] Beards G, 1995, J DIARRHOEAL DIS RES, V13, P235
  • [4] HUMAN AND BOVINE SEROTYPE G8 ROTAVIRUSES MAY BE DERIVED BY REASSORTMENT
    BROWNING, GF
    SNODGRASS, DR
    NAKAGOMI, O
    KAGA, E
    SARASINI, A
    GERNA, G
    [J]. ARCHIVES OF VIROLOGY, 1992, 125 (1-4) : 121 - 128
  • [5] CHANG LW, 1995, BIOMED ENG-APP BAS C, V7, P1
  • [6] Comparative amino acid sequence analysis of the outer capsid protein VP4 from four lapine rotavirus strains reveals identity with genotype P[14] human rotaviruses
    Ciarlet, M
    Estes, MK
    Conner, ME
    [J]. ARCHIVES OF VIROLOGY, 1997, 142 (05) : 1059 - 1069
  • [7] ROTAVIRUS ISOLATE WI61 REPRESENTING A PRESUMPTIVE NEW HUMAN SEROTYPE
    CLARK, HF
    HOSHINO, Y
    BELL, LM
    GROFF, J
    HESS, G
    BACHMAN, P
    OFFIT, PA
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (09) : 1757 - 1762
  • [8] NEUTRALIZING MONOCLONAL-ANTIBODIES TO HUMAN ROTAVIRUS AND INDICATIONS OF ANTIGENIC DRIFT AMONG STRAINS FROM NEONATES
    COULSON, BS
    FOWLER, KJ
    BISHOP, RF
    COTTON, RGH
    [J]. JOURNAL OF VIROLOGY, 1985, 54 (01) : 14 - 20
  • [9] Cunliffe NA, 1998, B WORLD HEALTH ORGAN, V76, P525
  • [10] Cunliffe NA, 1999, J MED VIROL, V57, P308, DOI 10.1002/(SICI)1096-9071(199903)57:3&lt