Biosynthesis of Antinutritional Alkaloids in Solanaceous Crops Is Mediated by Clustered Genes

被引：443

作者：

Itkin, M. ^{[1
]}

Heinig, U. ^{[1
]}

Tzfadia, O. ^{[1
]}

Bhide, A. J. ^{[1
,2
]}

Shinde, B. ^{[1
,2
]}

Cardenas, P. D. ^{[1
]}

Bocobza, S. E. ^{[1
]}

Unger, T. ^{[3
]}

Malitsky, S. ^{[1
]}

Finkers, R. ^{[4
]}

Tikunov, Y. ^{[4
]}

Bovy, A. ^{[4
]}

Chikate, Y. ^{[1
,2
]}

Singh, P. ^{[1
,2
]}

Rogachev, I. ^{[1
]}

Beekwilder, J. ^{[4
]}

Giri, A. P. ^{[1
,2
]}

Aharoni, A. ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Plant Sci, IL-76100 Rehovot, Israel

[2] CSIR, Natl Chem Lab, Div Biochem Sci, Plant Mol Biol Unit, Pune 411008, Maharashtra, India

[3] Weizmann Inst Sci, Israel Struct Prote Ctr, IL-76100 Rehovot, Israel

[4] Univ Wageningen & Res Ctr, NL-6700 AA Wageningen, Netherlands

来源：

SCIENCE | 2013年 / 341卷 / 6142期

基金：

欧洲研究理事会;

关键词：

GLYCOALKALOIDS; POTATO; PLANT; METABOLITES; PATHWAYS; SAPONINS; TOMATO;

D O I：

10.1126/science.1240230

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Steroidal glycoalkaloids (SGAs) such as alpha-solanine found in solanaceous food plants-as, for example, potato-are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.

引用

页码：175 / 179

页数：5

共 18 条

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