Identification of mandarin hybrids by isozyme and RAPD analysis

被引：45

作者：

Elisiário, PJ ^{[1
]}

Justo, EM ^{[1
]}

Leitao, JM ^{[1
]}

机构：

[1] Univ Algarve, UCTA, P-8000 Faro, Portugal

来源：

SCIENTIA HORTICULTURAE | 1999年 / 81卷 / 03期

关键词：

isozymes; RAPDs; Citrus; mandarins; DNA extraction;

D O I：

10.1016/S0304-4238(99)00013-8

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

A cross between the monoembryonic Clementine MA3 SRA 649 used as seed parent and the polyembryonic Carvalhais mandarin used as pollen progenitor was carried out in the SRA INRA-CIRAD, S. Giuliano, Corsica. Cuttings of 61 plants putatively resulting from this cross were sent to the Centro de Citricultura, Fare, Portugal for further agronomic evaluation under field trials. Isozyme analysis allowed the identification of 59 plants as having sexual origin, however, two plants that showed isozyme patterns identical to their seed parent were impossible to identify as being either of zygotic or of somatic origin. Isozymes confirmed Carvalhais mandarin as the pollen parent of 42 progeny plants, while RAPD analysis confirmed Carvalhais paternity over the remaining 19 plants. A fast and low pollutant method for extraction of Citrus total genomic DNA suitable for RAPD analysis is reported. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：287 / 299

页数：13

共 30 条

[1] DNA extraction and RAPD markers to assess the genetic similarity among Gelidium sesquipedale (Rhodophyta) populations [J].