Comparison between a liquid chromatography-tandem mass spectrometry assay and a fluorescent polarization immunoassay to measure whole blood everolimus concentration in heart and renal transplantations

被引：5

作者：

Dailly, Eric ^{[1
,2
]}

Deslandes, G. ^{[1
]}

Hourmant, M. ^{[3
]}

Petit, T. ^{[4
]}

Renaud, C. ^{[1
]}

Treilhaud, M.

Jolliet, P. ^{[1
,2
]}

机构：

[1] CHU Nantes, Hotel Dieu, Dept Clin Pharmacol, Nantes, France

[2] Rech Clin & Mesures Subject Sante, Fac Med Pharm, EA Biostat 4275, Nantes, France

[3] CHU Nantes, Hotel Dieu, Dept Nephrol & Clin Immunol, Nantes, France

[4] CHU Nantes, Laennec Hosp, Dept Cardiovasc Surg, Thorac Transplantat Unit, Nantes, France

来源：

JOURNAL OF CLINICAL LABORATORY ANALYSIS | 2008年 / 22卷 / 04期

关键词：

everolimus; chromatography; therapeutic drug monitoring; immunoassay;

D O I：

10.1002/jcla.20258

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Various methods [fluorescent polarization immunoassay (FPIA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay] are used for therapeutic drug monitoring of everolimus. The aim of this study is to compare these assays in renal and heart transplantation. The correlation between results was investigated by linear regression in 44 patients (24 heart recipients and 20 renal recipients-137 samples). The comparison between assays was performed by a paired t-test. A highly significant correlation was found between FPIA and LC-MS/MS in heart and renal recipients [FPlA = 0.851 x LC-MS/MS+ 1.773r(2) = 0.8738 (P<0.001)]. Paired t-tests did not show a significant difference between everolimus whole blood concentrations in the populations of heart and renal recipients or heart recipients or renal recipients. FPIA and LC-MS/MS assays gave consistent overall results although some significant differences were observed in some samples between these methods indicating that FPIA assay has limitations that deserve further investigations.

引用

页码：282 / 285

页数：4

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