The pentatricopeptide repeat gene OTP51 with two LAGLIDADG motifs is required for the cis-splicing of plastid ycf3 intron 2 in Arabidopsis thaliana

被引：134

作者：

de Longevialle, Andeol Falcon ^{[1
,2
]}

Hendrickson, Luke

Taylor, Nicolas L. ^{[1
]}

Delannoy, Etienne ^{[1
]}

Lurin, Claire ^{[2
]}

Badger, Murray ^{[3
]}

Millar, A. Harvey ^{[1
]}

Small, Ian ^{[1
,2
]}

机构：

[1] Univ Western Australia, Australian Res Council Ctr Excellence Plant Energ, Crawley, WA 6009, Australia

[2] Univ Evry Essonne, CNRS, INRA, Unite Mixte Rech Genom Vegetale, F-91057 Evry, France

[3] Australian Natl Univ, Australian Res Council Ctr Excellence Plant Energ, Canberra, ACT 0200, Australia

来源：

PLANT JOURNAL | 2008年 / 56卷 / 01期

基金：

澳大利亚研究理事会;

关键词：

PPR protein; RNA splicing; chloroplast; LAGLIDADG motif;

D O I：

10.1111/j.1365-313X.2008.03581.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The Arabidopsis thaliana chloroplast contains 20 group-II introns in its genome, and seven known splicing factors are required for the splicing of overlapping subsets of 19 of them. We describe an additional protein (OTP51) that specifically promotes the splicing of the only group-II intron for which no splicing factor has been described previously. This protein is a pentatricopeptide repeat (PPR) protein containing two LAGLIDADG motifs found in group-I intron maturases in other organisms. Amino acids thought to be important for the homing endonuclease activity of other LAGLIDADG proteins are missing in this protein, but the amino acids described to be important for maturase activity are conserved. OTP51 is absolutely required for the splicing of ycf3 intron 2, and also influences the splicing of several other group-IIa introns. Loss of OTP51 has far-reaching consequences for photosystem-I and photosystem-II assembly, and for the photosynthetic fluorescence characteristics of mutant plants.

引用

页码：157 / 168

页数：12

共 57 条

[1] The multifarious roles of PPR proteins in plant mitochondrial gene expression [J].

Andres, Charles ;

Lurin, Claire ;

Small, Ian D. .

PHYSIOLOGIA PLANTARUM, 2007, 129 (01) :14-22

[2] A CRM domain protein functions dually in group I and group II intron splicing in land plant chloroplasts [J].

Asakura, Yukari ;

Barkan, Alice .

PLANT CELL, 2007, 19 (12) :3864-3875

[3] Arabidopsis orthologs of maize chloroplast splicing factors promote splicing of orthologous and species-specific group II introns [J].

Asakura, Yukari ;

Barkan, Alice .

PLANT PHYSIOLOGY, 2006, 142 (04) :1656-1663

[4] Recruitment of intron-encoded and co-opted proteins in splicing of the bI3 group I intron RNA [J].

Bassi, GS ;

de Oliveira, DM ;

White, MF ;

Weeks, KM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (01) :128-133

[5] DETERMINATION OF THE QUANTUM EFFICIENCY OF PHOTOSYSTEM-II AND OF NONPHOTOCHEMICAL QUENCHING OF CHLOROPHYLL FLUORESCENCE IN THE FIELD [J].

BILGER, W ;

SCHREIBER, U ;

BOCK, M .

OECOLOGIA, 1995, 102 (04) :425-432

[6] Structural and biochemical analyses of DNA and RNA binding by a bifunctional homing endonuclease and group I intron splicing factor [J].

Bolduc, JM ;

Spiegel, PC ;

Chatterjee, P ;

Brady, KL ;

Downing, ME ;

Caprara, MG ;

Waring, RB ;

Stoddard, BL .

GENES & DEVELOPMENT, 2003, 17 (23) :2875-2888

[7] The ins and outs of group II introns [J].

Bonen, L ;

Vogel, J .

TRENDS IN GENETICS, 2001, 17 (06) :322-331

[8] Cis- and trans-splicing of group II introns in plant mitochondria [J].

Bonen, Linda .

MITOCHONDRION, 2008, 8 (01) :26-34

[9] The chloroplast ycf3 and ycf4 open reading frames of Chlamydomonas reinhardtii are required for the accumulation of the photosystem I complex [J].

Boudreau, E ;

Takahashi, Y ;

Lemieux, C ;

Turmel, M ;

Rochaix, JD .

EMBO JOURNAL, 1997, 16 (20) :6095-6104

[10] An allosteric-feedback mechanism for protein-assisted group I intron splicing [J].

Caprara, Mark G. ;

Chatterjee, Piyali ;

Solem, Amanda ;

Brady-Passerini, Kristina L. ;

Kaspar, Benjamin J. .

RNA, 2007, 13 (02) :211-222

← 1 2 3 4 5 6 →