Real-time detection and quantification of adenosine triphosphate sulfurylase activity by a bioluminometric approach

被引：11

作者：

Karamohamed, S ^{[1
]}

Nyrén, P ^{[1
]}

机构：

[1] Royal Inst Technol, Dept Biotechnol, SE-10044 Stockholm, Sweden

来源：

ANALYTICAL BIOCHEMISTRY | 1999年 / 271卷 / 01期

关键词：

luminescence; real-time; ATP; ATP sulfurylase; firefly luciferase; pyrophosphate;

D O I：

10.1006/abio.1999.4130

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

real-time, sensitive, and simple assay for detection and quantification of adenosine triphosphate sulfurylase (ATP:sulfate adenylytransferase, EC 2.7.7.4) activity has been developed. The method is based on detection of ATP generated in the ATP sulfurylase reaction between APS and PP, by the firefly luciferase system. For the Saccharomyces cerevisiae ATP sulfurylase, the concentrations of APS and PPI at the half-maximal rate were found to be about 0.5 and 7 mu M, respectively. The assay is sensitive and yields linear response between 0.1 mu U and 50 mU. The method can be used for monitoring and quantification of recombinant ATP sulfurylase activity in Escherichia coli lysate, as well as for detection of the activity during different purification procedures. (C) 1999 Academic Press.

引用

页码：81 / 85

页数：5

共 31 条

[1] NEW ASSAY FOR ATP-SULPHURYLASE USING LUCIFERIN-LUCIFERASE METHOD [J].

BALHARRY, GJ ;

NICHOLAS, DJ .

ANALYTICAL BIOCHEMISTRY, 1971, 40 (01) :1-&

[2] THE SACCHAROMYCES-CEREVISIAE MET3 GENE - NUCLEOTIDE-SEQUENCE AND RELATIONSHIP OF THE 5' NONCODING REGION TO THAT OF MET25 [J].

CHEREST, H ;

KERJAN, P ;

SURDINKERJAN, Y .

MOLECULAR & GENERAL GENETICS, 1987, 210 (02) :307-313

[3] ATP SULFURYLASE-DEPENDENT ASSAYS FOR INORGANIC PYROPHOSPHATE - APPLICATIONS TO DETERMINING THE EQUILIBRIUM-CONSTANT AND REVERSE DIRECTION KINETICS OF THE PYROPHOSPHATASE REACTION, MAGNESIUM BINDING TO ORTHO-PHOSPHATE, AND UNKNOWN CONCENTRATIONS OF PYROPHOSPHATE [J].

DALEY, LA ;

RENOSTO, F ;

SEGEL, IH .

ANALYTICAL BIOCHEMISTRY, 1986, 157 (02) :385-395

[4]

FOSTER BA, 1994, J BIOL CHEM, V269, P19777

[5] ADENOSINE 5'-TRIPHOSPHATE SULFURYLASE FROM SACCHAROMYCES-CEREVISIAE [J].

HAWES, CS ;

NICHOLAS, DJ .

BIOCHEMICAL JOURNAL, 1973, 133 (03) :541-550

[6] ENHANCEMENT OF THERMOSTABILITY OF FIREFLY LUCIFERASE FROM LUCIOLA-LATERALIS BY A SINGLE AMINO-ACID SUBSTITUTION [J].

KAJIYAMA, N ;

NAKANO, E .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1994, 58 (06) :1170-1171

[7] Production, purification, and luminometric analysis of recombinant Saccharomyces cerevisiae MET3 adenosine triphosphate sulfurylase expressed in Escherichia coli [J].

Karamohamed, S ;

Nilsson, J ;

Nourizad, K ;

Ronaghi, M ;

Pettersson, B ;

Nyrén, P .

PROTEIN EXPRESSION AND PURIFICATION, 1999, 15 (03) :381-388

[8] CHARACTERIZATION OF THE GENE ENCODING THE AUTOTROPHIC ATP SULFURYLASE FROM THE BACTERIAL ENDOSYMBIONT OF THE HYDROTHERMAL VENT TUBEWORM RIFTIA-PACHYPTILA [J].

LAUE, BE ;

NELSON, DC .

JOURNAL OF BACTERIOLOGY, 1994, 176 (12) :3723-3729

[9] CLONING OF A CDNA-ENCODING ATP SULFURYLASE FROM ARABIDOPSIS-THALIANA BY FUNCTIONAL EXPRESSION IN SACCHAROMYCES-CEREVISIAE [J].

LEUSTEK, T ;

MURILLO, M ;

CERVANTES, M .

PLANT PHYSIOLOGY, 1994, 105 (03) :897-902

[10]

LEYH TS, 1992, J BIOL CHEM, V267, P542

← 1 2 3 4 →