A colorimetric assay to evaluate the chemotherapeutic response of children with acute lymphoblastic leukemia (ALL) employing AchatininH:: a 9-O-acetyl sialic acid binding lectin

Employing a 9-O-acetyl sialic acid binding lectin, Achatinin(H) (ATN(H)), we have reported a non-invasive, blood based lymphoproliferation assay which measures the maximal lymphoproliferative dose (MLD) of ATN(H) to assess the status of 9-O-acetylated sialoglycoconjugates (9-OAcSGs) in patients with Acute lymphoblastic leukemia (ALL) (Mandal C, Sinha D, Sharma V, Bhattacharya DK. O-acetyl sialic acid binding lectin, as:a probe for detection of subtle changes on the cell surface induced during acute lymphoblastic leukemia [ALL] and its clinical application. Ind J Biochem Biophys 1997;34:82; Sinha D, Mandal C, Bhattacharya DK. Development of a simple blood based lymphoproliferation assay to assess the clinical status of patients with acute lymphoblastic leukemia. Leuk Res 1999;13:309-312; Sinha D, Mandal C, Bhattacharya DK. A novel method for prognostic evaluation of childhood acute lymphoblastic leukemia. Leukemia 1999;13[in press]). Although the expression of 9-OAcSGs clearly serves as an index of treatment outcome, the assay has limitations in that it requires radioisotopes, i.e. [H-3]-TdR. Therefore a colorimetric assay was developed as an alternative approach. The pre-treatment MLD, as measured by the colorimetric assay, was 0.15 +/- 0.02 mu g which progressively increased during consolidation therapy (1.40 +/- 0.39 mu g), maintenance therapy (4.20 +/- 1.60 mu g) and in followed-up cases (5.20 +/- 0.43 mu g) but sharply declined following relapse (0.25 +/- 0.02 mu g). The colorimetric assay also showed a good correlation with radiometric assay (r = +0.93) and their mean coefficient of inter-assay precision were also comparable (15.53% versus 14.86%. We therefore propose that the colorimetric assay is a safe, non-radiometric, user-friendly alternative for assessing individual chemotherapeutic responses in childhood ALL. (C) 1999 Elsevier Science Ltd. All rights reserved.