Glycosphingolipids are not pivotal receptors for Subtilase cytotoxin in vivo: Sensitivity analysis with glycosylation-defective mutant mice

被引：12

作者：

Kondo, Yuji ^{[1
]}

Tokuda, Noriyo ^{[1
]}

Fan, Xiayan ^{[1
]}

Yamashita, Tatsuyuki ^{[2
]}

Honke, Koichi ^{[2
]}

Takematsu, Hiroshi ^{[3
]}

Togayachi, Akira ^{[4
]}

Ohta, Michio ^{[5
]}

Kotzusumi, Yasunori ^{[3
]}

Narimatsu, Hisashi ^{[4
]}

Tajima, Orie ^{[1
,6
]}

Furukaw, Keiko ^{[1
,6
]}

Furukawa, Koichi ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Med, Dept Biochem 2, Showa Ku, Aichi 4660065, Japan

[2] Kochi Univ, Sch Med, Dept Mol Genet, Kochi 7838505, Japan

[3] Kyoto Univ, Grad Sch Biostudies, Lab Membrane Biochem & Biophys, Kyoto 6068501, Japan

[4] Natl Inst Adv Ind Sci & Technol, Res Ctr Med Glycosci, Tsukuba, Ibaraki 3058568, Japan

[5] Nagoya Univ, Grad Sch Med, Dept Microbiol, Showa Ku, Aichi 4660065, Japan

[6] Chubu Univ, Coll Life & Hlth Sci, Dept Biomed Sci, Kasugai, Aichi 4878501, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 378卷 / 02期

关键词：

Subtilase; Glycolipid; Toxin; Receptor; Knockout; AB(5); GANGLIOSIDES; BOTULINUM; IDENTIFICATION; EXPRESSION; CLONING; ENZYME;

D O I：

10.1016/j.bbrc.2008.10.163

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Certain glycosphingolipids play important roles as cellular receptor for bacterial toxins with high specificity and strong affinity. In particular AB(5) toxins exhibit typical modes of cell attachment with B5 and invasion and biological effects in cells with A subunit. Subtilase cytotoxin (SubAB) is the prototype of a recently discovered AB(5) cytotoxin family produced by certain strains of Shiga toxigenic Escherichia coli, and shows highly specific serine protease activity toward endoplasmic reticulum chaperone Bip Since. this toxin bound to a mimic of ganglioside GM2, GM2 has been considered to he possible receptor for SubAB. Using six kinds of glycosylation-defective knockout mice lacking certain group Of glycosphingolipids, sensitivity to SubAB in vivo was analyzed. Consequently, all mutant mice died at around 70 h after intraperitoneal injection of 10 mu g (or 7.5 mu g) of SubAB as well as wild type mice, These results indicated none of glycolipids are not pivotal receptor for SubAB in the body. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：179 / 181

页数：3

共 18 条

[1] Expression cloning of mouse cDNA of CMP-NeuAc:lactosylceramide α2,3-sialyltransferase, an enzyme that initiates the synthesis of gangliosides [J].