Immunolocalisation of PIVKA-II in paraffin-embedded specimens of hepatocellular carcinoma

被引：13

作者：

Tamano, M ^{[1
]}

Sugaya, H ^{[1
]}

Oguma, M ^{[1
]}

Murohisa, T ^{[1
]}

Tomita, Y ^{[1
]}

Matsumura, A ^{[1
]}

Kojima, K ^{[1
]}

Terano, A ^{[1
]}

机构：

[1] Dokkyo Univ, Sch Med, Dept Gastroenterol, Mibu, Tochigi 3210293, Japan

来源：

LIVER | 1999年 / 19卷 / 05期

关键词：

PIVKA-II; hepatocellular carcinoma; immunohistochemistry;

D O I：

10.1111/j.1478-3231.1999.tb00069.x

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Aims/Background: Although serum PIVKA-II has been widely used as a tumor marker for hepatocellular carcinoma (HCC), little information is available concerning tissue PIVKA-II, and detection of tissue PIVKA-II in paraffin-embedded tissue specimens of HCC is also considered difficult., Methods: Paraffin-embedded HCC tissues obtained at autopsy from 22 patients were subjected to immunohistochemical staining using the antibody MU-3 (Eisai Co., Ltd.) after microwave antigen retrieval. Results: PIVKA-II was mainly detected in the cytoplasm of HCC cells. The intensity of tissue PTVKA-II staining was not correlated with serum PTVKA-II levels or with the histological differentiation of HCC, However PIVKA-II staining tended to be more intense in tissue from patients with portal tumor thrombus, distant metastases, or a longer duration of HCC. Conclusion: This method of immunohistochemical staining is easy and simple to use and may be helpful for detecting tissue PIVKA-II in paraffin-embedded HCC specimens.

引用

页码：406 / 410

页数：5

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