Chemiluminescence assay of alkaline phosphatase using cortisol-21-phosphate as substrate and its application to enzyme immunoassays

被引：53

作者：

Kokado, A ^{[1
]}

Tsuji, A ^{[1
]}

Maeda, M ^{[1
]}

机构：

[1] SHOWA UNIV,SCH PHARMACEUT SCI,SHINAGAWA KU,TOKYO 142,JAPAN

来源：

ANALYTICA CHIMICA ACTA | 1997年 / 337卷 / 03期

关键词：

chemiluminescence; enzymatic methods; immunoassay; alkaline phosphatase; cortisol-21-phosphate; human chorionic gonadotropin; human growth hormone; alpha-fetoprotein; estradio;

D O I：

10.1016/S0003-2670(96)00400-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method is described for the highly sensitive chemiluminescence (CL) assay for alkaline phosphatase (ALP) using cortisol-21-phosphate (cortisol-21-P) as substrate and lucigenin as CL reagent; it is applied to an enzyme immunoassay (EIA) for human chorionic gonadotropin (hCG), human growth hormone (hGH), alpha-fetoprotein (AFP) and estradiol (E(2)). The detection limit for ALP was 8.75 x 10(-20) mol/assay, and the average coefficient of variation (n=11) over the calibration range (3.55 x 10(-15)-7.1 x 10(-10) moll(-1)) was 6.4%. In EIA for hCG, hGH, AFP and E(2), the detection limits were 0.008 mIU ml(-1),10, 40 and 0.25 pg ml(-1), respectively.

引用

页码：335 / 340

页数：6

共 17 条

[1] 1,2-DIOXETANES - NOVEL CHEMI-LUMINESCENT ENZYME SUBSTRATES - APPLICATIONS TO IMMUNOASSAYS [J].

BRONSTEIN, I ;

EDWARDS, B ;

VOYTA, JC .

JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE, 1989, 4 (01) :99-111

[2]

BRONSTEIN I, 1989, CLIN CHEM, V35, P1441

[3]

GEIGER R, 1987, J CLIN CHEM CLIN BIO, V25, P31

[4] Chemo-luminescence of dimethyl-diacridylium salts. [J].

Gleu, K ;

Petsch, W .

ANGEWANDTE CHEMIE, 1935, 48 :0057-0059

[5]

KAWAMOTO M, 1991, BUNSEKI KAGAKU, V40, P537

[6] A NEW HIGHLY SENSITIVE CHEMILUMINESCENT ASSAY OF ALKALINE-PHOSPHATASE USING LUCIGENIN AND ITS APPLICATION TO ENZYME-IMMUNOASSAY [J].

KITAMURA, M ;

MAEDA, M ;

TSUJI, A .

JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE, 1995, 10 (01) :1-7

[7] DETERMINATION OF CONJUGATED GLUCURONIC-ACID BY COMBINING ENZYMATIC-HYDROLYSIS WITH LUCIGENIN CHEMI-LUMINESCENCE [J].

KLOPF, LL ;

NIEMAN, TA .

ANALYTICAL CHEMISTRY, 1985, 57 (01) :46-51

[8]

MAEDA M, 1991, BIOLUMINESCENCE AND CHEMILUMINESCENCE, P119

[9]

Maeda M, 1986, ANAL SCI, V2, P183

[10]

MISKA W, 1987, J CLIN CHEM CLIN BIO, V25, P27

← 1 2 →