Expression of prolactin receptors in human osteosarcoma cells

被引：38

作者：

BatailleSimoneau, N ^{[1
]}

Gerland, K ^{[1
]}

Chappard, D ^{[1
]}

Basle, MF ^{[1
]}

Mercier, L ^{[1
]}

机构：

[1] UFR MED, LHEA, F-49045 ANGERS, FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 229卷 / 01期

关键词：

D O I：

10.1006/bbrc.1996.1800

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression of prolactin receptor (PRL-R) mRNA was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) combined with Southern analysis in total RNA extracts from two human osteosarcoma cell lines (MG-63 and Saos-2). The level of PRL-R transcript was significantly enhanced in cells cultured in the presence of 1,25-(OH)(2) vitaminD(3) (10(-7)M) and to a lesser extent in the presence of dexamethasone (10(-6)M). This first demonstration of PRL-R gene expression in osteoblastlike cells supports the hypothesis of a direct action of prolactin in bone cells, which is further strongly suggested by the stimulatory effect of 1,25-(OH)(2) vitaminD(3) and dexamethasone on PRL-R mRNA level in these cells. (C) 1996 Academic Press, Inc.

引用

页码：323 / 328

页数：6

共 26 条

[1] A NOVEL FAMILY OF GROWTH-FACTOR RECEPTORS - A COMMON BINDING DOMAIN IN THE GROWTH-HORMONE, PROLACTIN, ERYTHROPOIETIN AND IL-6 RECEPTORS, AND THE P75 IL-2 RECEPTOR BETA-CHAIN [J].