Analysis of thymic stromal cell populations using flow cytometry

被引：169

作者：

Gray, DHD ^{[1
]}

Chidgey, AP ^{[1
]}

Boyd, RL ^{[1
]}

机构：

[1] Monash Univ Sch Med, Dept Pathol & Immunol, Melbourne, Vic 3181, Australia

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2002年 / 260卷 / 1-2期

关键词：

thymus; stromal cells; flow cytometry; thymic epithelial cells;

D O I：

10.1016/S0022-1759(01)00493-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The complexity of the lymphostromal interplay that is essential to alphabetaT-cell development is reflected by the heterogeneity of both lymphocytes and thymic stromal cells. While panels of monoclonal antibodies have described many of the cellular components of these microenvironments, the means to quantify stromal cell subsets using flow cytometry remains poorly defined. This study refines and compares various stromal cell isolation procedures and determines the effects of various digestion enzymes on important surface molecules. Three- and four-color flow cytometry is used to correlate established and novel stromal cell markers to define thymic fibroblasts, epithelium and a unique subset of thymic endothelium that express MHC class II. This work provides a basis for the purification of thymic stromal cells for further phenotypic, functional and genetic analysis. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：15 / 28

页数：14

共 30 条

[1] Fibroblast dependency during early thymocyte development maps to the CD25(+) CD44(+) stage and involves interactions with fibroblast matrix molecules [J].

Anderson, G ;

Anderson, KL ;

Tchilian, EZ ;

Owen, JJT ;

Jenkinson, EJ .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1997, 27 (05) :1200-1206

[2] Cellular interactions in thymocyte development [J].

Anderson, G ;

Moore, NC ;

Owen, JJT ;

Jenkinson, EJ .

ANNUAL REVIEW OF IMMUNOLOGY, 1996, 14 :73-99

[3] POSITIVE SELECTION OF THE T-CELL REPERTOIRE - WHERE AND WHEN DOES IT OCCUR [J].

BENOIST, C ;

MATHIS, D .

CELL, 1989, 58 (06) :1027-1033

[4] THE THYMIC MICROENVIRONMENT [J].

BOYD, RL ;

TUCEK, CL ;

GODFREY, DI ;

IZON, DJ ;

WILSON, TJ ;

DAVIDSON, NJ ;

BEAN, AGD ;

LADYMAN, HM ;

RITTER, MA ;

HUGO, P .

IMMUNOLOGY TODAY, 1993, 14 (09) :445-459

[5]

Chidgey A P, 1998, Dev Immunol, V6, P157, DOI 10.1155/1998/10534

[6]

DEMAAGD RA, 1985, IMMUNOLOGY, V54, P745

[7]

FARR AG, 1985, J IMMUNOL, V134, P2971

[8]

Godfrey D I, 1988, Adv Exp Med Biol, V237, P269

[9] FLOW CYTOMETRIC ANALYSIS REVEALS UNEXPECTED SHARED ANTIGENS BETWEEN HISTOLOGICALLY DEFINED POPULATIONS OF THYMIC STROMAL CELLS [J].

IZON, DJ ;

NIELAND, JD ;

GODFREY, DI ;

BOYD, RL ;

KRUISBEEK, AM .

INTERNATIONAL IMMUNOLOGY, 1994, 6 (01) :31-39

[10] STUDIES ON T-CELL MATURATION ON DEFINED THYMIC STROMAL CELL-POPULATIONS INVITRO [J].

JENKINSON, EJ ;

ANDERSON, G ;

OWEN, JJT .

JOURNAL OF EXPERIMENTAL MEDICINE, 1992, 176 (03) :845-853

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