Fluorimetric detection of enzymatic activity associated with the human tumor suppressor Fhit protein

被引：5

作者：

Asensio, AC ^{[1
]}

Oaknin, S ^{[1
]}

Rotllán, P ^{[1
]}

机构：

[1] Univ La Laguna, Dept Bioquim & Biol Mol, E-38206 Tenerife, Canary Islands, Spain

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1999年 / 1432卷 / 02期

关键词：

Fhit; tumor suppressor; diadenosine triphosphate hydrolase; diadenosine; 5; '''-P-1; P-3-triphosphate; fluorogenic substrate; suramin;

D O I：

10.1016/S0167-4838(99)00102-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human tumor suppressor Fhit protein exhibits diadenosine triphosphatase activity, hydrolyzing Ap(3)A to AMP and ADP. We report that Fhit protein efficiently cleaves the fluorogenic Ap(3)A analog diethenoadenosine triphosphate giving support to establish a simple fluorimetric assay for quantification of Fhit enzyme. Fluorimetric assays were initially tested to demonstrate that diethyl pyrocarbonate and suramin inhibit Fhit enzyme. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：396 / 400

页数：5

共 20 条

[1] Baghdiguian S, 1997, CANCER J - FRANCE, V10, P31
[2] ASSAY OF DIADENOSINE TETRAPHOSPHATE HYDROLYTIC ENZYMES BY BORONATE CHROMATOGRAPHY
BARNES, LD
ROBINSON, AK
MUMFORD, CH
GARRISON, PN
[J]. ANALYTICAL BIOCHEMISTRY, 1985, 144 (01) : 296 - 304
[3] Fhit, a putative tumor suppressor in humans, is a dinucleoside 5',5'''-P-1,P-3-triphosphate hydrolase
Barnes, LD
Garrison, PN
Siprashvili, Z
Guranowski, A
Robinson, AK
Ingram, SW
Croce, CM
Ohta, M
Huebner, F
[J]. BIOCHEMISTRY, 1996, 35 (36) : 11529 - 11535
[4] LOCATION AND CHARACTERIZATION OF THE SURAMIN BINDING-SITES OF HUMAN SERUM-ALBUMIN
BOS, OJM
VANSTERKENBURG, ELM
BOON, JPCI
FISCHER, MJE
WILTING, J
JANSSEN, LHM
[J]. BIOCHEMICAL PHARMACOLOGY, 1990, 40 (07) : 1595 - 1599
[5] Purification and crystallization of complexes modeling the active state of the fragile histidine triad protein
Brenner, C
Pace, HC
Garrison, PN
Robinson, AK
Rosler, A
Liu, XH
Blackburn, GM
Croce, CM
Huebner, K
Barnes, LD
[J]. PROTEIN ENGINEERING, 1997, 10 (12): : 1461 - 1463
[6] Druck T, 1997, CANCER RES, V57, P504
[7] Greenspan DL, 1997, CANCER RES, V57, P4692
[8] REGIOSPECIFICITY OF THE HYDROLYSIS OF DIADENOSINE POLYPHOSPHATES CATALYZED BY 3 SPECIFIC PYROPHOSPHOHYDROLASES
GURANOWSKI, A
BROWN, P
ASHTON, PA
BLACKBURN, GM
[J]. BIOCHEMISTRY, 1994, 33 (01) : 235 - 240
[9] GURANOWSKI A, 1992, AP4A OTHER DINUCLEOS, P81
[10] Structure-based analysis of catalysis and substrate definition in the HIT protein family
Lima, CD
Klein, MG
Hendrickson, WA
[J]. SCIENCE, 1997, 278 (5336) : 286 - 290

← 1 2 →