Simultaneous analysis of γ-hydroxybutyric acid, γ-butyrolactone, and 1,4-butanediol by micellar electrokinetic chromatography

被引：19

作者：

Dahlén, J ^{[1
]}

Vriesman, T

机构：

[1] SKL, Natl Lab Forens Sci, SE-58194 Linkoping, Sweden

[2] Univ Strathclyde, Forens Sci Unit, Glasgow G1 1XW, Lanark, Scotland

来源：

FORENSIC SCIENCE INTERNATIONAL | 2002年 / 125卷 / 2-3期

关键词：

gamma-hydroxybutyric acid; gamma-butyrolactone; 1,4-butanediol; capillary electrophoresis; micellar electrokinetic chromatography;

D O I：

10.1016/S0379-0738(02)00006-3

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

A micellar electrokinetic chromatography (MEKC) method was optimised for simultaneous analysis of gamma-hydroxybutyric acid (GHB), gamma-butyrolactone (GBL), and 1,4-butanediol (BD). Best conditions for separation and baseline stability were achieved using a carrier electrolyte comprising 30.0 mM sodium barbital and 150.0 mM sodium dodecyl sulphate (SDS) at pH 10.2. Calibration functions were linear, giving correlation coefficients (r(2)) >0.998 for the three target compounds. Limits of detection (LOD) defined as three times the noise, were 5.1 mg/l, 0.34 and 0.25 g/l for GHB, GBL and BD, respectively. The repeatability of migration times and peak areas, expressed as the R.S.D. (n = 9) was better than 0.41 and 3.05%, respectively. Some casework samples were analysed using the optimised conditions. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：113 / 119

页数：7

共 30 条

[1] QUANTITATIVE-DETERMINATION OF ORGANIC-SOLVENTS BY CAPILLARY ELECTROPHORESIS USING INDIRECT UV DETECTION [J].

ALTRIA, KD ;

HOWELLS, JS .

JOURNAL OF CHROMATOGRAPHY A, 1995, 696 (02) :341-348

[2] INDIRECT FLUORESCENCE DETECTION IN MICELLAR ELECTROKINETIC CHROMATOGRAPHY [J].

AMANKWA, LN ;

KUHR, WG .

ANALYTICAL CHEMISTRY, 1991, 63 (17) :1733-1737

[3]

AUERBACH SB, 1990, MMWR-MORBID MORTAL W, V39, P861

[4] Fast separation of 16 seizure drug substances using non-aqueous capillary electrophoresis [J].

Bjornsdottir, I ;

Hansen, SH .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1999, 38 (02) :155-161

[5]

CHIN MY, 1992, WESTERN J MED, V156, P380

[6] Determination of γ-hydroxybutyrate (GHB) in biological specimens by cas chromatography -: Mass spectrometry [J].

Couper, FJ ;

Logan, BK .

JOURNAL OF ANALYTICAL TOXICOLOGY, 2000, 24 (01) :1-7

[7]

da Silva JAF, 2000, ELECTROPHORESIS, V21, P1405, DOI 10.1002/(SICI)1522-2683(20000401)21:7<1405::AID-ELPS1405>3.0.CO

[8]

2-O

[9] A novel method for GHB detection in urine and its application in drug-facilitated sexual assaults [J].

Elian, AA .

FORENSIC SCIENCE INTERNATIONAL, 2000, 109 (03) :183-187

[10] Identification and quantification of amphetamine and analogues by capillary zone electrophoresis [J].

Esseiva, P ;

Lock, E ;

Gueniat, O ;

Cole, MD .

SCIENCE & JUSTICE, 1997, 37 (02) :113-119

← 1 2 3 →