Antisense expression of an Arabidopsis Ran binding protein renders transgenic roots hypersensitive to auxin and alters auxin-induced root growth and development by arresting mitotic progress

被引:61
作者
Kim, SH
Arnold, D
Lloyd, A
Roux, SJ [1 ]
机构
[1] Univ Texas, Sect Mol Cell & Dev Biol, Austin, TX 78713 USA
[2] Univ Texas, Inst Cellular & Mol Biol, Austin, TX 78713 USA
[3] Stanford Univ, Dept Biol Sci, Herrin Lab, Stanford, CA 94305 USA
关键词
D O I
10.1105/tpc.13.12.2619
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We cloned a cDNA encoding an Arabidopsis Ran binding protein, AtRanBP1c, and generated transgenic Arabidopsis expressing the antisense strand of the AtRanBP1c gene to understand the in vivo functions of the Ran/RanBP signal pathway. The transgenic plants showed enhanced primary root growth but suppressed growth of lateral roots. Auxin significantly increased lateral root initiation and inhibited primary root growth in the transformants at 10 pM, several orders of magnitude lower than required to induce these responses in wild-type roots. This induction was followed by a blockage of mitosis in both newly emerged lateral roots and in the primary root, ultimately resulting in the selective death of cells in the tips of both lateral and primary roots. Given the established role of Ran binding proteins in the transport of proteins into the nucleus, these findings are consistent with a model in which AtRanBP1c plays a key role In the nuclear delivery of proteins that suppress auxin action and that regulate mitotic progress in root tips.
引用
收藏
页码:2619 / 2630
页数:12
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