Hypoxic Culture Conditions as a Solution for Mesenchymal Stem Cell Based Regenerative Therapy

被引:159
作者
Haque, Nazmul [1 ,2 ]
Rahman, Mohammad Tariqur [3 ]
Abu Kasim, Noor Hayaty [1 ,2 ]
Alabsi, Aied Mohammed [4 ,5 ]
机构
[1] Univ Malaya, Regenerat Dent Res Grp, Fac Dent, Kuala Lumpur 50603, Malaysia
[2] Univ Malaya, Dept Conservat Dent, Fac Dent, Kuala Lumpur 50603, Malaysia
[3] Int Islamic Univ Malaysia, Dept Biotechnol, Fac Sci, Kuantan 25200, Malaysia
[4] Univ Malaya, Dent Res & Training Unit, Fac Dent, Kuala Lumpur 50603, Malaysia
[5] Univ Malaya, Oral Canc Res & Coordinating Ctr, Fac Dent, Kuala Lumpur 50603, Malaysia
关键词
HUMAN BONE-MARROW; TISSUE OXYGEN-TENSION; STROMAL CELLS; GENE-EXPRESSION; OSTEOGENIC DIFFERENTIATION; TRANSENDOCARDIAL INJECTION; MYOCARDIAL-INFARCTION; PROMOTING MIGRATION; OXIDATIVE STRESS; PROGENITOR CELLS;
D O I
10.1155/2013/632972
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];
摘要
Cell-based regenerative therapies, based on in vitro propagation of stem cells, offer tremendous hope to many individuals suffering from degenerative diseases that were previously deemed untreatable. Due to the self-renewal capacity, multilineage potential, and immunosuppressive property, mesenchymal stem cells (MSCs) are considered as an attractive source of stem cells for regenerative therapies. However, poor growth kinetics, early senescence, and genetic instability during in vitro expansion and poor engraftment after transplantation are considered to be among the major disadvantages of MSC-based regenerative therapies. A number of complex inter-and intracellular interactive signaling systems control growth, multiplication, and differentiation of MSCs in their niche. Common laboratory conditions for stem cell culture involve ambient O-2 concentration (20%) in contrast to their niche where they usually reside in 2-9% O-2. Notably, O-2 plays an important role in maintaining stem cell fate in terms of proliferation and differentiation, by regulating hypoxia-inducible factor-1 (HIF-1) mediated expression of different genes. This paper aims to describe and compare the role of normoxia (20% O-2) and hypoxia (2-9% O-2) on the biology of MSCs. Finally it is concluded that a hypoxic environment can greatly improve growth kinetics, genetic stability, and expression of chemokine receptors during in vitro expansion and eventually can increase efficiency of MSC-based regenerative therapies.
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页数:12
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