An RNA helicase (AtSUV3) is present in Arabidopsis thaliana mitochondria

被引：26

作者：

Gagliardi, D

Kuhn, J

Spadinger, U

Brennicke, A

Leaver, CJ

Binder, S

机构：

[1] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England

[2] Univ Ulm, D-89069 Ulm, Germany

来源：

FEBS LETTERS | 1999年 / 458卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

plant mitochondrion; RNA metabolism; RNA helicase; RNA processing;

D O I：

10.1016/S0014-5793(99)01168-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The proteins involved in mitochondrial mRNA processing and degradation in higher plants have Set to be identified. As a first step towards this aim, me report here the characterisation of a nuclear-encoded DExH box RNA helicase (AtSUV3) localised in Arabidopsis thaliana mitochondria. The AtSUV3 mRNA is assembled from the 16 exons of a weakly expressed unique gene and the predicted protein has a calculated molecular weight of 63.6 kDa, Subcellular fractionation of transgenic plants expressing AtSUV3/GUS fusion proteins localises this protein in mitochondria. The N-terminal domain of AtSUV3 containing the motifs characteristic of DExH box RNA helicases exhibits a low endogenous ATPase activity in vitro which can be stimulated by the presence of mitochondrial RNA, confirming that AtSUV3 is an RNA helicase. (C) 1999 Federation of European Biochemical Societies.

引用

页码：337 / 342

页数：6

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