Cloning and sequencing of the cDNA encoding beta-glucosidase 1 from Aspergillus aculeatus

被引：39

作者：

Kawaguchi, T

Enoki, T

Tsurumaki, S

Sumitani, J

Ueda, M

Ooi, T

Arai, M

机构：

[1] UNIV OSAKA PREFECTURE, COLL AGR, DEPT APPL BIOL CHEM, OSAKA 593, JAPAN

[2] HOKKAIDO UNIV, DEPT MOL CHEM, HOKKAIDO 060, JAPAN

来源：

GENE | 1996年 / 173卷 / 02期

关键词：

nucleotide sequence; filamentous fungi; cDNA cloning;

D O I：

10.1016/0378-1119(96)00179-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A cDNA was isolated from an Aspergillus aculeatus cDNA library using synthetic oligodeoxyribonucleotide mixtures that corresponded to the internal amino acid (aa) sequence of mature beta-glucosidase 1 (BGL1). Analysis of the nucleotide sequence of the cloned cDNA insert revealed a 2580-bp open reading frame (ORF) that encoded a 860-aa protein. The deduced aa sequence of the ORF shared sequence similarity with several BGL from other microorganisms.

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收藏

页码：287 / 288

页数：2

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