Bifidobacterium longum, a lactic acid-producing intestinal bacterium inhibits colon cancer and modulates the intermediate biomarkers of colon carcinogenesis

被引:203
作者
Singh, J
Rivenson, A
Tomita, M
Shimamura, S
Ishibashi, N
Reddy, BS
机构
[1] AMER HLTH FDN, DIV NUTR CARCINOGENESIS, VALHALLA, NY 10595 USA
[2] AMER HLTH FDN, DIV PATHOL & TOXICOL, VALHALLA, NY 10595 USA
[3] MORINAGA MILK IND CO LTD, CTR RES & DEV, NUTR SCI LAB, ZAMA, KANAGAWA 228, JAPAN
关键词
D O I
10.1093/carcin/18.4.833
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The human colon can be described as a complex microbial ecosystem, comprising several hundred bacterial species, Some of these enteric bacteria are beneficial to the host and have been shown to exert antimutagenic and anticarcinogenic properties, We have investigated the colon tumor inhibitory activity of Bifidobacterium longum, a lactic acid-producing enterobacterium, The modifying effects of this lactic culture on colonic mucosal and/or tumor cell proliferation, ODC activity and ras-p21 oncoprotein expression in colon carcinogenesis were also analyzed. Male F344 rats were fed a modified AIN-76A diet containing 0 or 2% lyophilized cultures of B.longum and s.c. administered azoxymethane (AOM) dissolved in normal saline at a dose of 15 mg/kg body wt, once weekly for 2 weeks, Vehicle controls received an equal volume of normal saline s.c. Animals were maintained on control or experimental diets until termination of the study. Animals intended for analysis of cell proliferation were killed 20 weeks after the second AOM injection, whereas animals intended for colon tumor analysis and measurement of ODC activity and ras-p21 expression were killed 40 weeks after the last AOM injection, The data demonstrate that dietary administration of lyophilized cultures of B.longum resulted in significant suppression of colon tumor incidence and tumor multiplicity and also reduced tumor volume, Results also revealed that ingestion of B.longum significantly inhibited AOM-induced cell proliferation, ODC activity and expression of ras-p21 oncoprotein, Data suggest that oral administration of probiotic B.longum exerts strong antitumor activity, as indicated by modulation of the intermediate biomarkers of colon cancer, and consequently reduced tumor outcome.
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页码:833 / 841
页数:9
相关论文
共 90 条
[1]   INHIBITION OF INTESTINAL CARCINOGENESIS BY DIETARY SUPPLEMENTATION WITH CALCIUM [J].
APPLETON, GVN ;
DAVIES, PW ;
BRISTOL, JB ;
WILLIAMSON, RCN .
BRITISH JOURNAL OF SURGERY, 1987, 74 (06) :523-525
[2]   RAS ONCOGENES - THEIR ROLE IN NEOPLASIA [J].
BARBACID, M .
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 1990, 20 (03) :225-235
[3]  
BIASCO G, 1991, ITAL J GASTROENTEROL, V23, P142
[4]   EFFECT OF DIETARY CALCIUM AND CHOLIC-ACID ON THE PROLIFERATIVE INDEXES OF MURINE COLONIC EPITHELIUM [J].
BIRD, RP ;
SCHNEIDER, R ;
STAMP, D ;
BRUCE, WR .
CARCINOGENESIS, 1986, 7 (10) :1657-1661
[5]   ANTIMUTAGENIC ACTIVITY OF MILK FERMENTED BY STREPTOCOCCUS-THERMOPHILUS AND LACTOBACILLUS-BULGARICUS [J].
BODANA, AR ;
RAO, DR .
JOURNAL OF DAIRY SCIENCE, 1990, 73 (12) :3379-3384
[6]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[7]   MUTATIONS IN THE KRAS2 ONCOGENE DURING PROGRESSIVE STAGES OF HUMAN-COLON CARCINOMA [J].
BURMER, GC ;
LOEB, LA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (07) :2403-2407
[8]  
CAMPBELL TC, 1976, FED PROC, V35, P2470
[9]   A GENETIC MODEL FOR COLORECTAL TUMORIGENESIS [J].
FEARON, ER ;
VOGELSTEIN, B .
CELL, 1990, 61 (05) :759-767
[10]  
FIALA ES, 1977, CANCER, V40, P2436, DOI 10.1002/1097-0142(197711)40:5+<2436::AID-CNCR2820400908>3.0.CO