High performance liquid chromatographic method for the separation of isomers of cis- and trans-4-aminocyclohexylacetic acid and corresponding ethyl ester

被引：4

作者：

Maddox, NJ ^{[1
]}

Lin, S ^{[1
]}

机构：

[1] Warner Lambert Co, Parke Davis Pharmaceut Res Div, Chem Dev Dept, Holland, MI 49424 USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1999年 / 22卷 / 09期

关键词：

D O I：

10.1081/JLC-100101738

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Synthesis of PD 158771 (Figure 1) involved 4-aminocyclohexylacetic acid and its ethyl ester intermediates which required purification to obtain the desired corresponding transisomer. HPLC methods were developed for the separation of cis- and trans-4-aminocyclohexylacetic acid and the corresponding ethyl esters following pre-column derivatization with N alpha-(2,4-dinitro-5-fluorophenyl)-L-alaninamide (Marfey's reagent). The chromatographic conditions were varied to achieve optimal separation.

引用

页码：1367 / 1380

页数：14

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