Hydrochloric acid treatment for rapid recovery of Shiga toxin-producing Escherichia coli O26, O111 and O157 from faeces, food and environmental samples

被引：16

作者：

Fukushima, H ^{[1
]}

Gomyoda, M ^{[1
]}

机构：

[1] Publ Hlth Int Shimane Prefecture, Matsue, Shimane 6900122, Japan

来源：

ZENTRALBLATT FUR BAKTERIOLOGIE-INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY VIROLOGY PARASITOLOGY AND INFECTIOUS DISEASES | 1999年 / 289卷 / 03期

关键词：

D O I：

10.1016/S0934-8840(99)80066-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a hydrochloric acid treatment for the isolation of Shiga toxin-producing Escherichia coli (STEC) 026:H11, 0111:H- and O157:H7 strains from a variety of samples. After exposure to an equal volume of 1/8N HCl solution for 30 sec, the fecal suspensions and enrichment cultures were spread onto cefixime-tellurite-sorbitol-MacConkey (CT-SMAC) agar. This HCl treatment increased the sensitivity for detection of STEC 026:H11, 0111:H- and O157:H7 strains and decreased the growth of other microorganisms, from faecal samples and enrichment cultures of a variety of samples. This approach is an important economical and time-saving method to simplify and speed up isolation of STEC 026:H11, 0111:H- and O157:H7 from a variety of samples.

引用

页码：285 / 299

页数：15

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