New Methods for the Identification of Efflux Mediated MDR Bacteria, Genetic Assessment of Regulators and Efflux Pump Constituents, Characterization of Efflux Systems and Screening for Inhibitors of Efflux Pumps

被引：43

作者：

Viveiros, M.

Martins, M.

Couto, I. ^{[2
]}

Rodrigues, L.

Spengler, G.

Martins, A.

Kristiansen, J. E. ^{[3
]}

Molnar, J. ^{[4
]}

Amaral, L. ^{[1
]}

机构：

[1] Univ Nova Lisboa, UPMM, Unit Mycobacteriol, Inst Higiene & Med Trop, P-1349008 Lisbon, Portugal

[2] Univ Nova Lisboa, CREM, Fac Ciencias & Tecnol, P-28295169 Caparica, Portugal

[3] So Danish Univ, Dept Clin Microbiol, DK-6400 Sonderborg, Denmark

[4] Univ Szeged, Inst Med Microbiol, Dept Med Microbiol & Immunol, Szeged, Hungary

来源：

CURRENT DRUG TARGETS | 2008年 / 9卷 / 09期

关键词：

EB based methods; intrinsic efflux pumps; MDR efflux pumps; efflux pump inhibitors; MDR bacteria; fluorometric assay;

D O I：

10.2174/138945008785747734

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We have developed a number of methods that identify efflux pump mediated multi-drug resistant bacteria, characterize efflux systems and screen for inhibitors of efflux pumps. These approaches were complemented by the quantification of the expression of genes that regulate and code for constituents of efflux pumps. The methods described are easy to use, reproducible and for the most part, require instrumentation normally present in a clinical bacteriology laboratory. Because each method provides good reproducibility, they lend themselves for inter-laboratory use.

引用

页码：760 / 778

页数：19

共 79 条

[1] VceR negatively regulates the vceCAB MDR efflux operon and positively regulates its own synthesis in Vibrio cholerae 569B [J].