Novel block copolymer (PPDO/PLLA-b-PEG): Enhancement of DNA uptake and cell transfection

被引：18

作者：

Bhattarai, SR

Yi, HK

Bhattarai, N

Hwang, PH

Kim, HY

机构：

[1] Chonbuk Natl Univ, Dept Text Engn, Chonju 56756, Chhalabucdo, South Korea

[2] Chonbuk Natl Univ, Sch Med, Dept Pediat, Chonju, South Korea

[3] Univ Washington, Dept Mat Sci & Engn, Seattle, WA 98195 USA

[4] Chonbuk Natl Univ, Sch Dent, Dept Biochem, Chonju, South Korea

[5] Chonbuk Natl Univ, Dept Bionanosyst Engn, Chonju, South Korea

来源：

ACTA BIOMATERIALIA | 2006年 / 2卷 / 02期

关键词：

poly(p-dioxanone-co-L-lactide)-b-poly(ethylene glycol); biodegradable DNA; cell transfection; cell proliferation;

D O I：

10.1016/j.actbio.2005.10.007

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The cationic lipid mediated uptake of plasmid DNA by cells in monolayer culture was significantly enhanced with an aqueous solution of the block copolymer poly(p-dioxanone-CO-L-lactide)-b-poly(ethylene glycol) (PPDO/PLLA-b-PEG). Plasmid uptake studies with DNA encoding the beta-galactosidase gene and cytotoxicity evaluations were performed on MCF-7, NIH 3T3 and CT-26 cell lines. Transfection yields and time courses for maximum release of FITC labeled DNA in MCF-7 cells were observed and quantified by beta-galactosidase assay and spectrofluorometry, respectively. The reported results suggest that the studied block copolymer might be useful for the enhancement of polycation mediated transfection and could find application in gene therapy. (c) 2005 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：207 / 212

页数：6

共 13 条

[1] Hypersensitization of multidrug resistant human ovarian carcinoma cells by pluronic P85 block copolymer [J].