The endogenous sulfated pentapeptide phytosulfokine-α stimulates tracheary element differentiation of isolated mesophyll cells of zinnia

被引：92

作者：

Matsubayashi, Y ^{[1
]}

Takagi, L ^{[1
]}

Omura, N ^{[1
]}

Morita, A ^{[1
]}

Sakagami, Y ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Lab Bioact Nat Prod Chem, Nagoya, Aichi 4648601, Japan

来源：

PLANT PHYSIOLOGY | 1999年 / 120卷 / 04期

关键词：

D O I：

10.1104/pp.120.4.1043

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Dispersed zinnia (Zinnia elegans) mesophyll cells cannot differentiate into tracheary elements (TEs) at low cell density conditions even if auxin and cytokinin are present in the medium, indicating the involvement of intercellular interactions during the initiation and/or subsequent progresses in TE differentiation. When zinnia cells were incubated at a low density (2.5 x 10(4) cells mL(-1)) in TE-inductive medium in the presence of various concentrations of phytosulfokine (PSK)-alpha, which was originally identified as an intercellular signal peptide involved in cell proliferation, TE differentiation was strongly stimulated in a dose-dependent fashion; more than 35% of the living cells differentiated into TEs by 5 d of culture in the presence of 10 nM PSK-alpha. Enzyme-linked immunosorbent assay and mass spectroscopy confirmed that cultured zinnia cells produce nanomolar levels of PSKs under inductive conditions. These results suggest that PSK-alpha is a factor responsible for TE differentiation of zinnia mesophyll cells.

引用

页码：1043 / 1048

页数：6

共 16 条

[1] CONDITIONING FACTOR AFFECTING GROWTH IN PLANT-CELLS IN CULTURE [J].