Mutational analysis of the signal-sensing domain of ResE histidine kinase from Bacillus subtilis

被引:19
作者
Baruah, A [1 ]
Lindsey, B [1 ]
Zhu, Y [1 ]
Nakano, MM [1 ]
机构
[1] Oregon Hlth & Sci Univ, Dept Environm & Biomol Syst, OGI Sch Sci & Engn, Beaverton, OR 97006 USA
关键词
D O I
10.1128/JB.186.6.1694-1704.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Bacillus subtilis ResD-ResE two-component regulatory system activates genes involved in nitrate respiration in response to oxygen limitation or nitric oxide (NO). The sensor kinase ResE activates the response regulator ResD through phosphorylation, which then binds to the regulatory region of genes involved in anaerobiosis to activate their transcription. ResE is composed of an N-terminal signal input domain and a C-terminal catalytic domain. The N-terminal domain contains two transmembrane subdomains and a large extracytoplasmic loop. It also has a cytoplasmic PAS subdomain between the HAMP linker and C-terminal kinase domain. In an attempt to identify the signal-sensing subdomain of ResE, a series of deletions and amino acid substitutions were generated in the N-terminal domain. The results indicated that cytoplasmic ResE lacking the transmembrane segments and the extracytoplasmic loop retains the ability to sense oxygen limitation and NO, which leads to transcriptional activation of ResDE-dependent genes. This activity was eliminated by the deletion of the PAS subdomain, demonstrating that the PAS subdomain participates in signal reception. The study also raised the possibility that the extracytoplasmic region may serve as a second signal-sensing subdomain. This suggests that the extracytoplasmic region could contribute to amplification of ResE activity leading to the robust activation of genes required for anaerobic metabolism in B. subtilis.
引用
收藏
页码:1694 / 1704
页数:11
相关论文
共 43 条
[1]   Mutational analysis of a conserved signal-transducing element:: the HAMP linker of the Escherichia coli nitrate sensor NarX [J].
Appleman, JA ;
Stewart, V .
JOURNAL OF BACTERIOLOGY, 2003, 185 (01) :89-97
[2]   SUGARS INDUCE THE AGROBACTERIUM VIRULENCE GENES THROUGH A PERIPLASMIC BINDING-PROTEIN AND A TRANSMEMBRANE SIGNAL PROTEIN [J].
CANGELOSI, GA ;
ANKENBAUER, RG ;
NESTER, EW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (17) :6708-6712
[3]   Role of the periplasmic domain of the Escherichia coli NarX sensor-transmitter protein in nitrate-dependent signal transduction and gene regulation [J].
Cavicchioli, R ;
Chiang, RC ;
Kalman, LV ;
Gunsalus, RP .
MOLECULAR MICROBIOLOGY, 1996, 21 (05) :901-911
[4]   Mg2+ sensing by the Mg2+ sensor PhoQ of Salmonella enterica [J].
Chamnongpol, S ;
Cromie, M ;
Groisman, EA .
JOURNAL OF MOLECULAR BIOLOGY, 2003, 325 (04) :795-807
[5]   FUNCTIONAL ROLES ASSIGNED TO THE PERIPLASMIC, LINKER, AND RECEIVER DOMAINS OF THE AGROBACTERIUM-TUMEFACIENS VIRA PROTEIN [J].
CHANG, CH ;
WINANS, SC .
JOURNAL OF BACTERIOLOGY, 1992, 174 (21) :7033-7039
[6]   IDENTIFICATION AND CHARACTERIZATION OF NARQ, A 2ND NITRATE SENSOR FOR NITRATE-DEPENDENT GENE-REGULATION IN ESCHERICHIA-COLI [J].
CHIANG, RC ;
CAVICCHIOLI, R ;
GUNSALUS, RP .
MOLECULAR MICROBIOLOGY, 1992, 6 (14) :1913-1923
[7]   Two-component signal transduction in Bacillus subtilis:: How one organism sees its world [J].
Fabret, C ;
Feher, VA ;
Hoch, JA .
JOURNAL OF BACTERIOLOGY, 1999, 181 (07) :1975-1983
[8]   Redox signal transduction by the ArcB sensor kinase of Haemophilus influenzae lacking the PAS domain [J].
Georgellis, D ;
Kwon, O ;
Lin, ECC ;
Wong, SM ;
Akerley, BJ .
JOURNAL OF BACTERIOLOGY, 2001, 183 (24) :7206-7212
[9]   Quinones as the redox signal for the Arc two-component system of bacteria [J].
Georgellis, D ;
Kwon, O ;
Lin, ECC .
SCIENCE, 2001, 292 (5525) :2314-2316
[10]   Structure of a biological oxygen sensor: A new mechanism for heme-driven signal transduction [J].
Gong, WM ;
Hao, B ;
Mansy, SS ;
Gonzalez, G ;
Gilles-Gonzalez, MA ;
Chan, MK .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (26) :15177-15182