Effects of low-intensity pulsed ultrasound on proliferation and chondroitin sulfate synthesis of cultured chondrocytes embedded in Atelocollagen® gel

被引:39
作者
Nishikori, T [1 ]
Ochi, M [1 ]
Uchio, Y [1 ]
Maniwa, S [1 ]
Kataoka, H [1 ]
Kawasaki, K [1 ]
Katsube, K [1 ]
Kuriwaka, M [1 ]
机构
[1] Shimane Med Univ, Dept Orthopaed, Izumo, Shimane 6938501, Japan
来源
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH | 2002年 / 59卷 / 02期
关键词
ultrasound; chondrocyte; culture; atelocollagen((R)); transplantation;
D O I
10.1002/jbm.1226
中图分类号
R318 [生物医学工程];
学科分类号
0831 [生物医学工程];
摘要
The effects of low-intensity pulsed ultrasound (US) on the proliferation and chondroitin sulfate synthesis of cultured chondrocytes embedded in Atelocollagen (R) gel in vitro were examined. Articular cartilage was harvested from the hip, knee, and shoulder joints of 10-week-old Japanese white rabbits. Chondrocytes isolated by collagenase digestion were embedded in type I collagen gel, Atelocollagen gel, and were cultured in Dulbecco's modified eagle's medium for 3 weeks. The US apparatus, SAFHS (R), was used to deliver an ultrasound signal with spatial and temporal average intensities of 30 mW/cm(2) (US group). The frequency was 1.5 MHz with a 200-microsecond tone burst repeated at 1.0 kHz. US treatments were administered for 20 min per day under culture dishes, with the medium replaced twice a week. Another group of cells was exposed to sham ultrasound as a control. Cell number, histological findings, synthesis of isomers of chondroitin sulfate, and stiffness of the chondrocyte-collagen gel composites were analyzed. US exposure promoted synthesis of chondroitin sulfate, especially chondroitin 6-sulfate, although it did not significantly enhance cell number and stiffness. In this three-dimensional culture model, these results suggest that US exposure may be clinically useful in improving the quality of chondrocyte-Atelocollagen implants for transplantation into articular cartilage defects. (C) 2001 John Wiley & Sons, Inc.
引用
收藏
页码:201 / 206
页数:6
相关论文
共 40 条
[1]
RIB PERICHONDRIAL GRAFTS FOR THE REPAIR OF FULL-THICKNESS ARTICULAR-CARTILAGE DEFECTS - A MORPHOLOGICAL AND BIOCHEMICAL-STUDY IN RABBITS [J].
AMIEL, D ;
COUTTS, RD ;
ABEL, M ;
STEWART, W ;
HARWOOD, F ;
AKESON, WH .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1985, 67A (06) :911-920
[2]
REPAIR OF ARTICULAR SURFACES BY ALLOGRAFTS OF ARTICULAR AND GROWTH-PLATE CARTILAGE [J].
ASTON, JE ;
BENTLEY, G .
JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME, 1986, 68 (01) :29-35
[3]
AZUMA Y, 2000, ORTHOP SURG TRAUMATO, V43, P247
[4]
Chondroitin sulphation in human joint tissues varies with age, zone and topography [J].
Bayliss, MT ;
Davidson, C ;
Woodhouse, SM ;
Osborne, DJ .
ACTA ORTHOPAEDICA SCANDINAVICA, 1995, 66 :22-25
[5]
TREATMENT OF DEEP CARTILAGE DEFECTS IN THE KNEE WITH AUTOLOGOUS CHONDROCYTE TRANSPLANTATION [J].
BRITTBERG, M ;
LINDAHL, A ;
NILSSON, A ;
OHLSSON, C ;
ISAKSSON, O ;
PETERSON, L .
NEW ENGLAND JOURNAL OF MEDICINE, 1994, 331 (14) :889-895
[6]
DEW TL, 1992, AM J VET RES, V53, P2141
[7]
THE STIMULATION OF BONE-GROWTH BY ULTRASOUND [J].
DUARTE, LR .
ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY, 1983, 101 (03) :153-159
[8]
BIOCHEMICAL-STUDIES ON REPAIR CARTILAGE RESURFACING EXPERIMENTAL DEFECTS IN THE RABBIT KNEE [J].
FURUKAWA, T ;
EYRE, DR ;
KOIDE, S ;
GLIMCHER, MJ .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1980, 62 (01) :79-89
[9]
THE REPAIR OF EXPERIMENTALLY PRODUCED DEFECTS IN RABBIT ARTICULAR-CARTILAGE BY AUTOLOGOUS CHONDROCYTE TRANSPLANTATION [J].
GRANDE, DA ;
PITMAN, MI ;
PETERSON, L ;
MENCHE, D ;
KLEIN, M .
JOURNAL OF ORTHOPAEDIC RESEARCH, 1989, 7 (02) :208-218
[10]
ACCELERATION OF TIBIAL FRACTURE-HEALING BY NONINVASIVE, LOW-INTENSITY PULSED ULTRASOUND [J].
HECKMAN, JD ;
RYABY, JP ;
MCCABE, J ;
FREY, JJ ;
KILCOYNE, RF .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1994, 76A (01) :26-34