Rapid diagnosis of Staphylococcus aureus bacteremia using S-aureus PNA FISH

被引：37

作者：

González, V ^{[1
]}

Padilla, E ^{[1
]}

Giménez, M ^{[1
]}

Vilaplana, C ^{[1
]}

Pérez, A ^{[1
]}

Fernández, G ^{[1
]}

Quesada, MD ^{[1
]}

Pallarés, MA ^{[1
]}

Ausina, V ^{[1
]}

机构：

[1] Hosp Badalona Germans Trias & Pujol, Microbiol Serv, Barcelona 08916, Spain

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2004年 / 23卷 / 05期

关键词：

D O I：

10.1007/s10096-004-1112-x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

In the study presented here, the performance of the S. aureus PNA FISH assay was evaluated using 285 blood cultures (from 104 patients) that had gram-positive cocci resembling staphylococci on Gram stain. The new molecular test is based on a fluorescence in situ hybridization assay using peptide nucleic acid probes targeting Staphylococcus aureus 16S rRNA and is designed for the rapid identification of Staphylococcus aureus directly from positive blood cultures. The sensitivity, specificity, and positive and negative predictive values of the S. aureus PNA FISH for the rapid identification of Staphylococcus aureus directly from positive blood culture bottles were 100, 99.4, 99.2 and 100%, respectively.

引用

页码：396 / 398

页数：3

共 8 条

[1] Influence of antimicrobial treatment on mortality in septicemia [J].

Behrendt, G ;

Schneider, S ;

Brodt, HR ;

Just-Nübling, G ;

Shah, PM .

JOURNAL OF CHEMOTHERAPY, 1999, 11 (03) :179-186

[2]

BOUZA E, 1999, CLIN MICROBIOL INFEC, V5, P1

[3]

Drobniewski FA, 2000, J CLIN MICROBIOL, V38, P444

[4] Direct identification of Staphylococcus aureus from positive blood culture bottles [J].

Oliveira, K ;

Brecher, SM ;

Durbin, A ;

Shapiro, DS ;

Schwartz, DR ;

De Girolami, PC ;

Dakos, J ;

Procop, GW ;

Wilson, D ;

Hanna, CS ;

Haase, G ;

Peltroche-Llacsahuanga, H ;

Chapin, KC ;

Musgnug, MC ;

Levi, MH ;

Shoemaker, C ;

Stender, H .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (02) :889-891

[5] Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes [J].

Oliveira, K ;

Procop, GW ;

Wilson, D ;

Coull, J ;

Stender, H .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (01) :247-251

[6] Evaluation of a fluorescence hybridisation assay using peptide nucleic acid probes for identification and differentiation of tuberculous and non-tuberculous mycobacteria in liquid cultures [J].

Padilla, E ;

Manterola, JM ;

Rasmussen, OF ;

Lonca, J ;

Domínguez, J ;

Matas, L ;

Hernández, A ;

Ausina, V .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2000, 19 (02) :140-145

[7] Fluorescence in situ hybridization with peptide nucleic acid probes for rapid identification of Candida albicans directly from blood culture bottles [J].

Rigby, S ;

Procop, GW ;

Haase, G ;

Wilson, D ;

Hall, G ;

Kurtzman, C ;

Oliveira, K ;

Von Oy, S ;

Hyldig-Nielsen, JJ ;

Coull, J ;

Stender, H .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (06) :2182-2186

[8]

Stender H, 1999, INT J TUBERC LUNG D, V3, P830

← 1 →