Invasion potential of H22 hepatocarcinoma cells is increased by HMGB1-induced tumor NF-κB signaling via initiation of HSP70

被引:62
作者
Gong, Wei [1 ]
Wang, Zhi-Yong [2 ]
Chen, Guo-Xi [3 ]
Liu, Yu-Qiao [1 ]
Gu, Xiao-Yan [1 ]
Liu, Wen-Wei [4 ]
机构
[1] Hubei Univ Arts & Sci, XiangYang Cent Hosp, Dept Oncol, Xiangyang 441021, Hubei, Peoples R China
[2] Hubei Univ Arts & Sci, XiangYang Cent Hosp, Dept Neurosurg, Xiangyang 441021, Hubei, Peoples R China
[3] Wuhan TB Prevent & Cure Inst, Dept Med Res, Wuhan 430010, Hubei, Peoples R China
[4] Hubei Univ Arts & Sci, XiangYang Cent Hosp, Dept Cardiol, Xiangyang 441021, Hubei, Peoples R China
基金
美国国家科学基金会;
关键词
heat shock protein 70; high-mobility group protein B1; Toll-like receptor 2/4; tumor invasion; NF-kappa B; MOBILITY GROUP BOX-1; TRANSDUCTION PATHWAY; IMMUNE-RESPONSE; RECEPTOR TLR; CANCER; RELEASE; HMGB1; GROWTH; DEATH; INFLAMMATION;
D O I
10.3892/or.2013.2595
中图分类号
R73 [肿瘤学];
学科分类号
100214 [肿瘤学];
摘要
The functional relationship and cross-regulation between damage-associated molecular patterns and NF-kappa B in the tumor microenvironment remains unclear. In the present study, high-mobility group protein B1 (HMGB1) was secreted in response to feed second phase of NF-kappa B activation from heat shock protein (HSP) 70 that may result in a higher invasion potential of hepatocarcinoma cells. HSP70 promoted the proliferation of H22 hepatocarcinoma cells through Toll-like receptor (TLR) 2 and TLR4 signaling and induced the early phosphorylation of NF-kappa B, which reached maximum levels within 30 min. However, HSP70 promoted the upregulation of Beclin-1 expression via Jun N-terminal kinase (JNK) activation in tumor cells and the release of HMGB1 from tumor cells. Inhibition of Beclin-1/c-JNK production prevented the second, but not the first, phase of NF-kappa B phosphorylation, implicating Beclin-1/c-JNK in the second phase of phosphorylation. HSP70 induced Beclin-1-derived HMGB1 production at 4 h, which occurred before the rise in the second phosphorylation that occurred at 6 h. Exogenous HMGB1 also induced the rapid phosphorylation of NF-kappa B and upregulated the expression of MMP-9, inhibited the rapid phosphorylation of NF-kappa B and reduced MMP-9 by receptor for advanced glycation end products (RAGE) inhibitor that prevented HMGB1-induced cell invasion in vitro, which demonstrated that the biological significance of HMGB1/RAGE is key to the second, but not the first, phase of NF-kappa B phosphorylation in tumor cells. HSP70 triggered a positive feedback loop of NF-kappa B activation in H22 cells. The second phase of NF-kappa B phosphorylation mediated by HSP70 is implicated in the increase of tumor cell malignant invasion.
引用
收藏
页码:1249 / 1256
页数:8
相关论文
共 29 条
[1]
Toll-like receptor 4-dependent contribution of the immune system to anticancer chemotherapy and radiotherapy [J].
Apetoh, Lionel ;
Ghiringhelli, Francois ;
Tesniere, Antoine ;
Obeid, Michel ;
Ortiz, Carla ;
Criollo, Alfredo ;
Mignot, Gregoire ;
Maiuri, M. Chiara ;
Ullrich, Evelyn ;
Saulnier, Patrick ;
Yang, Huan ;
Amigorena, Sebastian ;
Ryffel, Bernard ;
Barrat, Franck J. ;
Saftig, Paul ;
Levi, Francis ;
Lidereau, Rosette ;
Nogues, Catherine ;
Mira, Jean-Paul ;
Chompret, Agnes ;
Joulin, Virginie ;
Clavel-Chapelon, Francoise ;
Bourhis, Jean ;
Andre, Fabrice ;
Delaloge, Suzette ;
Tursz, Thomas ;
Kroemer, Guido ;
Zitvogel, Laurence .
NATURE MEDICINE, 2007, 13 (09) :1050-1059
[2]
Novel signal transduction pathway utilized by extracellular HSP70 -: Role of Toll-like receptor (TLR) 2 AND TLR4 [J].
Asea, A ;
Rehli, M ;
Kabingu, E ;
Boch, JA ;
Baré, O ;
Auron, PE ;
Stevenson, MA ;
Calderwood, SK .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (17) :15028-15034
[3]
Asea Alexzander, 2006, Current Immunology Reviews, V2, P209, DOI 10.2174/157339506778018514
[4]
Necrotic but not apoptotic cell death releases heat shock proteins, which deliver a partial maturation signal to dendritic cells and activate the NF-κB pathway [J].
Basu, S ;
Binder, RJ ;
Suto, R ;
Anderson, KM ;
Srivastava, PK .
INTERNATIONAL IMMUNOLOGY, 2000, 12 (11) :1539-1546
[5]
Alternative mechanism by which IFN-γ enhances tumor recognition:: Active release of heat shock protein 72 [J].
Bausero, MA ;
Gastpar, R ;
Multhoff, G ;
Asea, A .
JOURNAL OF IMMUNOLOGY, 2005, 175 (05) :2900-2912
[6]
Masquerader: High mobility group box-1 and cancer [J].
Ellerman, Jessica E. ;
Brown, Charles K. ;
de Vera, Michael ;
Zeh, Herbert J. ;
Billiar, Timothy ;
Rubartelli, Anna ;
Lotze, Michael T. .
CLINICAL CANCER RESEARCH, 2007, 13 (10) :2836-2848
[7]
HSP70 vaccine in combination with gene therapy with plasmid DNA encoding sPD-1 overcomes immune resistance and suppresses the progression of pulmonary metastatic melanoma [J].
Geng, H ;
Zhang, GM ;
Xiao, H ;
Yuan, Y ;
Li, D ;
Zhang, H ;
Qiu, H ;
He, YF ;
Feng, ZH .
INTERNATIONAL JOURNAL OF CANCER, 2006, 118 (11) :2657-2664
[8]
IFN-γ withdrawal after immunotherapy potentiates B16 melanoma invasion and metastasis by intensifying tumor integrin αvβ3 signaling [J].
Gong, Wei ;
Zhang, Gui-Mei ;
Liu, Yi ;
Lei, Zhang ;
Li, Dong ;
Yuan, Ye ;
Huang, Bo ;
Feng, Zuo-Hua .
INTERNATIONAL JOURNAL OF CANCER, 2008, 123 (03) :702-708
[9]
Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling (Publication with Expression of Concern) [J].
Huang, Bo ;
Zhao, Jie ;
Shen, Shiqian ;
Li, Hongxing ;
He, Kai-Li ;
Shen, Guan-Xin ;
Mayer, Lloyd ;
UnkelesS, Jay ;
Li, Dong ;
Yuan, Ye ;
Zhang, Gui-Mei ;
Xiong, Huabao ;
Feng, Zuo-Hua .
CANCER RESEARCH, 2007, 67 (09) :4346-4352
[10]
Escaping cell death:: Survival proteins in cancer [J].
Jäättelä, M .
EXPERIMENTAL CELL RESEARCH, 1999, 248 (01) :30-43