Reversal of 5-flouroucial resistance by adenovirus-mediated transfer of wild-type p53 gene in multidrug-resiatant human colon carcinoma LoVo/5-FU cells

被引：7

作者：

Yu, Zhi-Wei ^{[1
]}

Zhao, Peng ^{[1
]}

Liu, Ming ^{[1
]}

Dong, Xin-Shu ^{[1
]}

Tao, Ji ^{[2
]}

Yao, Xue-Qin ^{[3
]}

Yin, Xin-Hua ^{[4
]}

Li, Yu ^{[5
]}

Fu, Song-Bin ^{[5
]}

机构：

[1] Harbin Med Univ, Affiliated Hosp 3, Dept Abdominal Surg, Harbin 150040, Heilongjiang Pr, Peoples R China

[2] Harbin Med Univ, Hosp 5, Dept Gen Surg, Harbin 150040, Heilongjiang Pr, Peoples R China

[3] Nanfang Hosp, Dept Gen Surg, Guangzhou 510515, Guangdong, Peoples R China

[4] Harbin Med Univ, Affiliated Hosp 2, Dept Geriatr, Harbin 150096, Heilongjiang Pr, Peoples R China

[5] Harbin Med Univ, Dept Mol Biol, Harbin 150057, Heilongjiang Pr, Peoples R China

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2004年 / 10卷 / 13期

关键词：

D O I：

暂无

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To observe the reversal effects of wide-type p53 gene on multi-drug resistance to 5-FU (LOVO/5-FU). METHODS: After treatment with Ad-p53, LOVO/5-FU sensitivity to 5-Fu was investigated using tetrazolium dye assay. Multidrug resistance gene-1 (MDR1) gene expression was assayed by semi-quantitative reverse transcription-polymerase chain reaction and the expression of p53 protein was examined by Western blotting. RESULTS: The reversal activity after treatment with wide-type p53 gene was increased up to 4.982 fold at 48 h. The expression of MDR1 gene decreased significantly after treatment with wide-type p53 gene, and the expression of p53 protein lasted for about 5 d, with a peak at 48 h, and began to decrease at 72 h. CONCLUSION: Wide-type p53 gene has a remarkable reversal activity for the high expression of MDR1 gene in colorectal cancers. The reversal effects seem to be in a time dependent manner. It might have good prospects in clinical application.

引用

页码：1979 / 1983

页数：5

共 38 条

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