Recombination of nanometric vesicles during freeze-drying

被引：11

作者：

Cabane, B

Blanchon, S

Neves, C

机构：

[1] PMMH, ESPCI, F-75231 Paris 05, France

[2] Paris Res Ctr, Dept Pharmaceut Sci, F-94400 Vitry Sur Seine, France

来源：

LANGMUIR | 2006年 / 22卷 / 05期

关键词：

D O I：

10.1021/la051923g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Concentrated dispersions of nanometric lipid vesicles (mean diameter 20 nm) in water/maltose solutions have been freeze-dried and then redispersed in water, yielding again dispersions of lipid vesicles. At each stage of the freeze-drying process, the organization of the vesicles in the dispersion and their size distribution were examined through small-angle neutron scattering and gel permeation chromatography. It was found that the osmotic deswelling of the vesicles caused them to recombine into larger vesicles. A single burst of recombination events occurred when the maltose concentration in the aqueous phase rose above 100 g/L. The final vesicle population was monopopulated, with a central diameter about twice as large as that of the original dispersion.

引用

收藏

页码：1982 / 1990

页数：9

相关论文

共 24 条

[1] EFFECT OF CARBOHYDRATES AND GLYCEROL ON THE STABILITY AND SURFACE-PROPERTIES OF LYOPHILIZED LIPOSOMES [J].

ALONSOROMANOWSKI, S ;

BIONDI, AC ;

DISALVO, EA .

JOURNAL OF MEMBRANE BIOLOGY, 1989, 108 (01) :1-11

[2] THE PROTECTIVE EFFECT OF FREE AND MEMBRANE-BOUND CRYOPROTECTANTS DURING FREEZING AND FREEZE-DRYING OF LIPOSOMES [J].

AUSBORN, M ;

SCHREIER, H ;

BREZESINSKI, G ;

FABIAN, H ;

MEYER, HW ;

NUHN, P .

JOURNAL OF CONTROLLED RELEASE, 1994, 30 (02) :105-116

[3]

AUSBORN M, 1992, EUR J PHARM BIOPHARM, V38, P133

[4] CONTROLLED ENVIRONMENT VITRIFICATION SYSTEM - AN IMPROVED SAMPLE PREPARATION TECHNIQUE [J].

BELLARE, JR ;

DAVIS, HT ;

SCRIVEN, LE ;

TALMON, Y .

JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE, 1988, 10 (01) :87-111

[5] PRESERVATION OF DRY LIPOSOMES DOES NOT REQUIRE RETENTION OF RESIDUAL WATER [J].

CROWE, JH ;

SPARGO, BJ ;

CROWE, LM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (06) :1537-1540

[6] IS VITRIFICATION SUFFICIENT TO PRESERVE LIPOSOMES DURING FREEZE-DRYING [J].

CROWE, JH ;

LESLIE, SB ;

CROWE, LM .

CRYOBIOLOGY, 1994, 31 (04) :355-366

[7] PRESERVATION OF FREEZE-DRIED LIPOSOMES BY TREHALOSE [J].

CROWE, LM ;

CROWE, JH ;

RUDOLPH, A ;

WOMERSLEY, C ;

APPEL, L .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1985, 242 (01) :240-247

[8] FREEZE-DRYING OF LIPOSOMES WITH FREE AND MEMBRANE-BOUND CRYOPROTECTANTS - THE BACKGROUND OF PROTECTION AND DAMAGING PROCESSES [J].

ENGEL, A ;

BENDAS, G ;

WILHELM, F ;

MANNOVA, M ;

AUSBORN, M ;

NUHN, P .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1994, 107 (02) :99-110

[9] CRITICAL PARAMETERS IN FREEZING OF LIPOSOMES [J].

FRANSEN, GJ ;

SALEMINK, PJM ;

CROMMELIN, DJA .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1986, 33 (1-3) :27-35

[10] Characterization of loaded liposomes by size exclusion chromatography [J].

Grabielle-Madelmont, C ;

Lesieur, S ;

Ollivon, M .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2003, 56 (1-3) :189-217