Selective solid-phase extraction sorbent for caffeine made by molecular imprinting

被引：133

作者：

Theodoridis, G ^{[1
]}

Manesiotis, P ^{[1
]}

机构：

[1] Aristotelian Univ Thessaloniki, Dept Chem, Analyt Chem Lab, GR-54006 Thessaloniki, Greece

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 948卷 / 1-2期

关键词：

molecular imprinting; solid-phase extraction; caffeine; theophylline; theobromine; xanthines;

D O I：

10.1016/S0021-9673(01)01457-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A molecularly imprinted polymer (MIP) was prepared with caffeine as the template molecule. Thermal polymerisation (60degreesC) was optimised, varying ratios of monomer, cross linker and template. The polymer was used as a solid-phase extraction (SPE) sorbent, for selective trapping and pre-concentration of caffeine. Caffeine was loaded on the MIP-SPE cartridge using different loading conditions (solvents, pH value), Washing and elution of the caffeine bound to the MIP was studied utilising different protocols. The extraction protocol was successfully applied to the direct extraction of caffeine from beverages and spiked human plasma. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：163 / 169

页数：7

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