A starvation-induced noncoding RNA modulates expression of Dicer-regulated genes

被引：60

作者：

Hellwig, Sabine

Bass, Brenda L. ^{[1
]}

机构：

[1] Univ Utah, Dept Biochem, Salt Lake City, UT 84112 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2008年 / 105卷 / 35期

基金：

美国国家卫生研究院;

关键词：

adenosine deaminase that acts on RNA (ADAR); Caenorhabditis elegans; double-stranded RNA; miRNA; siRNA;

D O I：

10.1073/pnas.0805118105

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Although much has been learned about short noncoding RNAs, long noncoding transcripts are largely uncharacterized. Here, we describe Caenorhabditis elegans rncs-1, a highly base-paired, 800-nucleotide noncoding RNA expressed in hypodermis and intestine. Transcription of rncs-1 is modulated in response to food supply. Although highly double-stranded, we show that rncs-1 RNA is not a substrate for Dicer because of branched structures at its termini. However, rncs-1 RNA inhibits Dicer cleavage of a second dsRNA in vitro, presumably by competition. We validate this observation in vivo by demonstrating that mRNA levels of several Dicer-regulated genes vary with changes in rncs-1 expression. Certain viruses express dsRNA to compete with cellular dsRNA-mediated pathways, and our data suggest that rncs-1 provides a cellular correlate of this phenomenon.

引用

页码：12897 / 12902

页数：6

共 32 条

[1] Suppression of RNA interference by adenovirus virus-associated RNA [J].