Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism (AFLP) and directed amplification of minisatellite region DNA (DAMD)

被引：56

作者：

Ha, WY

Shaw, PC

Liu, J

Yau, FCF

Wang, J ^{[1
]}

机构：

[1] Chinese Univ Hong Kong, Dept Biochem, Shatin, Hong Kong, Peoples R China

[2] Chinese Univ Hong Kong, Inst Chinese Med, Shatin, Hong Kong, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2002年 / 50卷 / 07期

关键词：

Panax ginseng; Panax quinquefolius; AFLP; DAMD;

D O I：

10.1021/jf011365l

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

AFLP profiles characteristic to Panax ginseng and Panax quinquefolius were generated using primers E-AGG/M-CAA. P. ginseng samples from different farms in China and Korea are homogeneous genetically [similarity index (SI) = 0.88-0.99], whereas samples of P. quinquefolius from different sources are much more heterogeneous (SI = 0.64-0.96). Detailed analysis of one of the polymorphic bands in P. ginseng led to the identification of a minisatellite Pg2, which contains eight repeats of 5'-AGGACTCATCACATTGTTACTC. The minisatellite DNA was consequently used in directed amplification minisatellite region DNA analysis to authenticate the two ginsengs.

引用

页码：1871 / 1875

页数：5

共 17 条

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