β-1,3-glucan reduces growth of Mycobacterium tuberculosis in macrophage cultures

被引:19
作者
Hetland, G
Sandven, P
机构
[1] Natl Inst Publ Hlth, Dept Environm Med, N-0403 Oslo, Norway
[2] Natl Inst Publ Hlth, Dept Bacteriol, Oslo, Norway
来源
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2002年 / 33卷 / 01期
关键词
beta-1,3-D-glucan; antibacterial effect; Mycobacterium tuberculosis; macrophage;
D O I
10.1111/j.1574-695X.2002.tb00570.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The effect of beta-1,3-D-glucans SSG, from Sclerotinia sclerotiorum, or soluble (sMG) or particulate (pMG) MacroGard((R)) from baker's yeast on growth of Mycobacterium tuberculosis H37Rv in cultures of peritoneal macrophages from BALB/c mice was examined. After 24 h intracellular bacteria from lysed macrophages were cultured and the number of cfu counted. SSG given with challenge, but not 24 h after, reduced the number of M. tuberculosis cfu significantly. pMG, but not sMG, given with challenge had an even stronger inhibitory effect, which was enhanced after serum opsonization of the particles. The effect of serum-treated pMG was abrogated by addition of a monoclonal antibody to CD11b. The results indicate that beta-glucans inhibit growth of M. tuberculosis in host cells in vitro, probably due to cellular stimulation and/or competitive inhibition of uptake of bacteria via CR3 (CD11b/18). (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:41 / 45
页数:5
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