Development of up-converting phosphor technology-based lateral-flow assay for rapidly quantitative detection of hepatitis B surface antibody

被引：63

作者：

Li, Luping ^{[1
,2
]}

Zhou, Lei ^{[3
]}

Yu, Yang ^{[2
]}

Zhu, Ziwen ^{[3
]}

Lin, Changqing ^{[4
]}

Lu, Changlong ^{[1
]}

Yang, Ruifu ^{[3
]}

机构：

[1] China Med Univ, Dept Immunol, Shenyang 110001, Peoples R China

[2] Shenyang Hosp Infect Dis, Shenyang 110006, Peoples R China

[3] Beijing Inst Microbiol & Epidemiol, Lab Analyt Microbiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China

[4] Beijing Hotgen Biotechnol Co Ltd, Beijing 100071, Peoples R China

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2009年 / 63卷 / 02期

关键词：

Up-converting phosphor technology; Biosensor; Lateral-flow strip; Hepatitis B surface antibody; Clinical quantitative detection; CHRONIC-RENAL-FAILURE; HEALTH-CARE WORKERS; VIRUS-INFECTION; C VIRUS; VACCINE; IMMUNOGENICITY; ANTIGEN; IMMUNOASSAY; REPORTERS; CHILDREN;

D O I：

10.1016/j.diagmicrobio.2008.10.020

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

An up-converting phosphor technology-based lateral-flow (UPT-LF) assay system was developed for rapid and quantitative detection of hepatitis B surface antibody (HBsAb). To evaluate its performance, we compared it with the Abbott Axsym AUSAB (ABBOTT Diagnostics Division, Wiesbaden, Germany) assay and conventional ELISA (Wantai Biological Pharmacy Enterprise, Beijing, China) using 13 standard positive sera and 306 clinical sera. In both laboratory evaluation and clinical application, UPT-LF assay showed the best sensitivity (99.19%) and detection agreement (97.43% for the adjusted agreement) with true results. The concordance rate between UPT-LF and ELISA, as shown by correlative regression analysis, was the highest (R-2 = 0.6389), whereas that between UPT-LF and AUSAB was the lowest (R-2 = 0.5702). In conclusion, UPT-LF assay for quantitative detection of HBsAb is sensitive and rapid, promising this new assay a bright future. (c) 2009 Published by Elsevier Inc.

引用

页码：165 / 172

页数：8

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